Mycobacterial reporter strains and uses thereof

Details Mycobacterial reporter strains and uses thereof Mycobacterial reporter strains and uses thereof

1994-10-03

1997-10-21

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 6, 435 8, 435 32, 4352523, 4352531, 4353201, C12Q 168, C12Q 166, C12Q 118, C12N 120

Patent

active

056795157

ABSTRACT:
This invention relates to a method of quantifying bacteria in vivo or in vitro using bacterial reporter strains. In particular this invention provides a method utilizing mycobacterial reporter strains that permits rapid screening for in vivo antimycobacterial activity of various compositions. In addition this invention provides for particular mycobacterial reporter strains expressing the FFlux gene at levels sufficiently high to allow detection in tissue homogenates without lysis or concentration of the bacteria.

REFERENCES:
patent: 5283179 (1994-02-01), Wood
patent: 5476768 (1995-12-01), Pearson et al.
Letters in Applied Microbiology, No. 19, No. 5, issued Nov. 1994, Gordon et al., "The application of luciferase as a reporter of environmental regulation of gene expression in mycobacteria", pp. 336-340, see p. 337.
Antimicrobial Agents and Chemotherapy, vol. 39, No. 3, issued Mar. 1995, Cooksey et al., "Bioluminescence Method to Evaluate Antimicrobial Agents against Mycobacterium avium", pp. 754-756, see p. 754.
Molecular Microbiology, vol. 15, No. 6, issued Feb. 1995, "L5 luciferase reporter mycobacteriophages: a sensitive tool for the detection and assay of live mycobacteria", pp. 1055-1067, see pp. 1056-1062.
Andrew, P.W. et al. "Construction of a Bioluminescent Mycobacterium and Its Use for Assay of Antimycobacterial Agents", Journal of Clinical Microbiology 31:2251 (1993).
Brasier, A.R. et al., "Optimized Use of the Firefly Luciferase Assay as a Reporter Gene in Mammalian Cell Lines.", BioTechniques Nov.-Dec.; 7(10):1116 (1989).
Cooksey, R.C., et al., "A Rapid Method for Screening Antimicrobial Agents for Activities against a Strain of Mycobacterium tuberculosis Expressing Firefly Luciferase." Antimicrobial Agents and Chemotherapy 37:1348 (1993).
DiLella, A.G. et al., "Utility of firefly luciferase as a reporter gene for promoter activity in transgenic mice." Nucleic Acids Res. May 11:16(9):4159 (1988).
Engebrecht, J. et al., "Measuring Gene Expression with Light." Science Mar 15;227(4692):1345 (1985).
Hanberger, H. et al., "Post-Antibiotic Effect of Beta-Lactam Antibiotics on Gram-Negative Bacteria in Relation to Morphology, Initial Killing and MIC." Eur. J. Clin. Microbiol. Infect. Dis. Nov;10(11):927 (1991).
Isaksson, B. et al., "Postantibiotic effect of aminoglycosides on staphylococci." J. Antimicrob. Chemother. Aug;32(2):215 (1993).
Isaksson, B. et al., "Synergic post-antibiotic effect of amikacin in combination with beta-lactam antibiotics on Gram-negative bacteria." J. Antimicrob. Chemother. Jul;28(1):25-34 (1991).
Jacobs, Jr., W.R. et al., "Rapid Assessment of Drug Susceptibilities of Mycobacterium tuberculosis by Means of Luciferase Reporter Phages." Science 260:819 (1993).
Jassim, S.A.A. et al., "In vivo Bioluminescence: A Cellular Reporter for Research and Industry." J. Biolumin. Chemilumin. Apr.-Jun.;5(2):115 (1990).
Kawamura, I. et al., "Enhanced protection of cyclophosphamide-treated mice against infection with Pseudomonas aeruginosa after treatment with Z-100, a polysaccharide-rich extract from Mycobacterium tuberculosis Aoyama B.", Immunopharmacol. Immunotoxicol. 12(3):331 (1990).
Korpela, M. et al., "Stable-light-emitting Escherichia coli as a Biosensor." J. Biolumin. Chemilumin July;4(1):551 (1989).
Limb, D.I. et al., "Comparison of techniques for antimicrobial susceptibility testing of mycobacteria." J. Clin. Pathol. May;46(5):403 (1993).
Schmetterer, G. et al., "Expression of Luciferases from Vibrio harveyi and Vibrio fischeri in Filamentous Cyanobacteria." J. Bacteriol. July;167(1):411 (1986).
Stewart, Lett. Appl. Microbiol. 10:1 (1990).
Stover et al., Nature, 351:456 (1991).
Stover et al., "Development of BCG Vaccines as a Live Recombinant Vaccine Vehicle." In Vaccines 91. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1991).
Wood, K.V. et al., "Introduction to Beetle Luciferases and their Applications." J. Biolumn Chemilumin July;4(1):289 (1989).
Jacobs et al., Science, vol. 260: 819-822, 1993.
DiLella et al., Nucleic Acids Research, vol. 16, No. 9: 4159, 1988.

Affiliated with

Also associated with

Rating

Mycobacterial reporter strains and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Mycobacterial reporter strains and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mycobacterial reporter strains and uses thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1005733