Mycobacterial genes down-regulated during latency

Details Mycobacterial genes down-regulated during latency Mycobacterial genes down-regulated during latency

2002-10-21

2010-10-12

Swartz, Rodney P. (Department: 1645)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Bacterium or component thereof or substance produced by said...

C424S009100, C424S009200, C424S184100, C424S185100, C424S190100, C424S234100, C435S004000, C435S007100, C435S007200, C530S300000, C530S350000

Reexamination Certificate

active

07811588

ABSTRACT:
A method is provided for identifying mycobacterial genes the expression of which is down-regulated during a stationary phase culture of mycobacteria under nutrient-starving conditions when compared with an exponential phase culture of mycobacteria under culture conditions that are not nutrient-starving and that support exponential growth of said mycobacteria. The described method optionally provides for identifying mycobacterial genes that are simultaneously down-regulated under low dissolved oxygen tension conditions. The down-regulated genes of the present invention form the basis of nucleic acid vaccines, or provide targets to allow preparation of attenuated mycobacteria for vaccines against mycobacterial infections. Similarly, peptides encoded by said down-regulated genes are employed in vaccines. In a further embodiment, the identified genes/peptides provide the means for identifying the presence of a mycobacterial infection in a clinical sample by nucleic acid probe or antibody detection.

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