Mycobacterial antigens expressed during latency

Details Mycobacterial antigens expressed during latency Mycobacterial antigens expressed during latency

2002-07-04

2008-07-01

Swartz, Rodney P (Department: 1645)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Bacterium or component thereof or substance produced by said...

C530S300000, C530S350000, C424S009100, C424S009200, C424S184100, C424S185100, C424S190100, C424S234100

Reexamination Certificate

active

07393540

ABSTRACT:
A method is provided for identifying mycobacterial genes that are induced or up-regulated under culture conditions that are nutrient-starving and which maintain mycobacterial latency, said conditions being obtainable by batch fermentation of amicobacteriumfor at least 20 days post-inoculation, when compared with culture conditions that are not nutrient-starving and which support exponential growth of saidmycobacterium. Said induced or up-regulated genes form the basis of nucleic acid vaccines, or provide targets to allow preparation of attenuatedmycobacteriafor vaccines against mycobacterial infections. Similarly, peptides encoded by said induced or up-regulated genes are employed in vaccines. In a further embodiment, the identified genes/peptides provide the means for identifying the presence of a mycobacterial infection in a clinical sample by nucleic acid probe or antibody detection.

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