MxA as an antiviral drug and as a target for identification...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C424S130100, C424S070100, C435S069100, C435S252300, C435S320100, C435S325000

Reexamination Certificate

active

07393941

ABSTRACT:
The invention features methods and compositions for use in a screening assay to identify agents having antiviral activity against a DNA virus by assessing the effect of a candidate agent upon alternative splicing of MxA, or by assessing the effect of a candidate agent on production of a variant form of MxA protein. The invention also provides methods for enhancing resistance of cells to infection by a DNA virus by providing for elevated MxA and/or by providing for reduced production of variant MxA protein.

REFERENCES:
patent: 2004/0209800 (2004-10-01), Mushinski et al.
Horisberger et al., Interferon-Induced Human Protein MxA Is a GTPase Which Binds Transiently to Cellular Proteins, 1992, Journal of Virology, vol. 66, No. 8, pp. 4705-4709.
Fernandez et al., Hepatitis B virus downregulates the human interferon-inducible MxA promoter through direct interaction of precore/core proteins, 2003, Journal of General Virology, vol. 84, pp. 2073-2082.
Asano et al., Polymorphisms and the Antiviral Property of Mx1 protein, 2002, Journal of Veterinary Medican Science, vol. 64, No. 12, pp. 1085-1089.
Aebi et al., “cDNA Structures and Regulation of Two Interferon-Induced Human Mx Proteins”, (1989), Moleular and Cellular Biology, vol. 9, No. 11, pp. 5062-5072.
Barreca et al., “Suppression of herpes simplex virus I in MDBK cells via the interferon pathway”, (2004), Journal of Virology, vol. 78, No. 16, pp. 8641-8653.
Flohr et al., “The central interactive region of human MxA GTPase is involved in GTPase activation and interaction with viral target structures”, (1999) Federation of European Biochemical Society, Letters 463, pp. 24-28.
Gordien et al., “Inhibition of Hepatitis B Virus Replication by the Interferon-Inducible MxA Protein”, (2001), Journal of Virology, vol. 75, No. 6, pp. 2684-2691.
Jatiani et al., “Expression of the antiviral protein MxA in cells transiently pertubs endocutosis”(2004), Biochemical and Biophysical Research Communications, vol. 323, pp. 541-546.
Mossman et al., “Herpes Simplex virus triggers and then disarms a host antiviral response”, (2001), Journal of Virology, vol. 75, No. 2, pp. 750-758.
Noisakran et al. “Type I interferons and herpes simplex virus infection: a naked DNA approach as a therapeutic option”, (2001), Immunol Research, vol. 24, No. 1, pp. 1-11.
Punda-Polic ET al. “Synergistic anti varicella-zosater virus activity of interferon-alpha 2a and acyclovir in corneal cells”, (1999), Zentralblatt fur Bakteriologie, vol. 289, No. 2, pp. 203-210.

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