Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1987-03-12
1989-01-17
Kight, John
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530412, 530417, 530350, 530351, 435 68, 435183, C07K 318
Patent
active
047988860
ABSTRACT:
A protein and a derivative thereof having a methionine residue added at the amino terminus are mutually separated with the utilization of the difference in affinity for hydroxyapatite between them, such as by means of liquid chromatography using a hydroxyapatite column.
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Protein Purification, ed Scopes, 1982, pp. 138-139.
Yamaha et al. CA vol. 104, 1986, #205032z.
Moriya et al., CA vol. 105, #207347a.
Yamada et al., Biochem. Biophys. Res. Comm., 135: 837-843 (Mar. 28, 1986).
Moriya et al., Bio/Technology, 4: 904-905 (Oct. 1986).
Kawasaki et al., Eur. J. Biochem. 152: 361-371 (1985).
Bernardi, "Chromatography of Proteins on Hydroxyapatite", Methods in Enzymology 27: 471-479 (1973).
Bernardi; "Chromatography of Proteins on Hydroxyapatite", Methods in Enzymology, vol. 22, pp. 325-339 (1971).
Kato Koichi
Kawahara Kenji
Yamada Takao
Conlin David G.
Draper Garnette D.
Kight John
Takeda Chemical Industries Ltd.
Williams Stephan P.
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