Muteins of TNF pharmaceutical compositions and a method of makin

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

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435 691, 435 695, 435 697, 4352523, 43525233, 4353201, 53395, 53402, C12N 1500, C12N 1509, A61K 3819

Patent

active

055191193

ABSTRACT:
A polypeptide which is a tumor necrosis factor polypeptide having an amino acid sequence represented by from the 1st Ser to the 155th Leu as shown by SEQ ID NO:1 in the Sequence Listing, or its mutein, wherein the amino acid sequence of the 1st Ser to the 8th Asp of the SEQ ID NO:1 or the corresponding amino acid sequence of the mutein is replaced by an amino acid sequence containing at least one amino acid sequence of Arg-Gly-Asp and from 3 to 16 amino acids. Also disclosed are a recombinant plasmid containing a DNA encoding such a polypeptide, a recombinant microbial cell transformed by such a recombinant plasmid, a process for producing the polypeptide, a pharmaceutical composition comprising the polypeptide as an active ingredient, and a DNA for the polypeptide.

REFERENCES:
patent: 4948875 (1990-08-01), Tanaka et al.
patent: 4990455 (1991-02-01), Yamagishi et al.
patent: 5028420 (1991-07-01), Masegi et al.
patent: 5180811 (1993-01-01), Doerper et al.
Malik Cancer Lit Abstract 00979234 1991.
Van Ostade Nature 361:266-269 1993.
Ruoslahti et al Cancer Cells 1(4):119-126 1989.

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