Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-11-01
1996-02-20
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 18, 435 691, 4351721, 435188, 435207, 435963, 930240, 935 14, C12N 938, C12N 1556, G01N 33535
Patent
active
054928133
ABSTRACT:
Muteins of enzyme acceptor polypeptide fragments of .beta.-galactosidase are provided which exhibit substantially increased kinetic complementation activity with no significant loss in stability. A preferred enzyme acceptor fragment has an amino acid other than cysteine located at position 500 of the natural sequence. An especially preferred substitution is serine or valine. Other preferred muteins have an amino acid other than methionine located at position 443, with leucine being especially preferred, or an amino acid other than cysteine at position 76, with leucine being an especially preferred substitution. Also provided are methods for producing the novel muteins, reagent compositions comprising the novel muteins, and immunoassay methods for determining an analyte in which the novel mutein recombines with an enzyme donor polypeptide fragment to form enzymatically active .beta.-galactosidase.
REFERENCES:
patent: 5120653 (1992-06-01), Henderson
Boguslawski Sophie J.
Eisenbeis Scott J.
Krevolin Mark
Ledden David J.
Amick Marilyn L.
Boehringer Mannheim Corporation
Kenemore Max J.
Saunders David
Young D. Michael
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