Mutations of the MDR1 P-glycoprotein that improves its...

Details Mutations of the MDR1 P-glycoprotein that improves its... Mutations of the MDR1 P-glycoprotein that improves its...

2007-12-18

2007-12-18

Landsman, Robert (Department: 1646)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S006120, C435S320100, C530S350000, C536S023500

Reexamination Certificate

active

10343657

ABSTRACT:
The present invention provides mutant P-glycoprotein polypeptides that confer increased resistance to certain chemotherapeutic drugs relative to wild-type P-glycoprotein or P-glycoprotein having a glycine to valine substitution at position 185, and nucleic acid molecules encoding the same. The invention also provides antibodies that specifically bind mutant P-glycoproteins. The invention further provides methods for the diagnosis and treatment of conditions associated with P-glycoprotein-mediated multidrug resistance.

REFERENCES:
patent: 5830697 (1998-11-01), Sikic et al.
Currier Stephen et al, Identification of residues in the first cytoplasmic loop of P-glycoprotein involved in the function of chimeric human MDR1-MDR2 transporters. Journ. of Biol. Chem. 267(35)25153-25159 (1992).
Roninson et al., Isolation of Altered-Function Mutants and Genetic Suppressor Elements of Multidrug Transporter P-Glycoprotein by Expression Selection from Retroviral Libraries. Methods in Enzymology 292:225-48 (1998).
Safa et al., “Molecular Basis of Preferential Resistance to Colchicine in Multidrug-Resistant Human Cell Conferred by GLY-185-Val-185 Substitution in P Glycoprotein” Proceeding of the National Academy of Sciences of USA 87(18) 7225-9 (Sep. 1990).
Ruth Adam et al., “Coordinate changes in drug resistance and drug-induced conformational transitions in altered-fuction mutants of the multidrug transporter P-glycoprotein” Biochem. 40(14)4332-9 (Apr. 10, 2001).
Loo et al., “Mutations to Amino Acids Located in Predicted Transmembrane Segment 6 (TM6) Modulate the Activity and Substrate Specificity of Human P-Glycoprotein,” Biochemistry 33(47)14049-57 (Nov. 1994).
Loo et al., Functional consequences of glycine mutations in the predicted cytoplasmic loops of P-glycoprotein Journ of Biol. Chem. 269(10) 7243-48 (Mar. 11, 1994).
Taguchi et al., “Alteration of substrate specifically by mutations at the His-61 position in predicted transmembrane domain 1 of human MDRI/P-glycoprotein” Biochemistry 36(29)8883-9 (1997).
Choi et al., “An Altered Pattern of Cross-Resistance in Multidrug-Resistant Human cells Results from Spontaneous Mutations in the mdr1 (P-Glycoprotein) Gene” Cell 53:519-29 (May 20, 1998).
Chen et al., “Internal Duplication and Homology with Bacterial Transport Proteins in the Mdr1 (P-Glycoprotein) Gene from Multidrug-Resistant Human Cells” Cell 47:381-89 (Nov. 7, 1986).

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