Mutation of repA protein

Details Mutation of repA protein Mutation of repA protein

1994-05-20

1996-12-10

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4352523, 4353201, 536 237, C12N 121, C12N 1531, C12N 1570

Patent

active

055830404

ABSTRACT:
Mutant plasmids of Rts1 and P1 are provided which permit for high copy numbers of the plasmid in host bacteria. The mutant plasmids also possess other improved characteristics, including the ability to coexist with other vectors in host bacteria, such as E. coli, the ability to control copy numbers, within the range of about 20 to 120, by the use of different host bacteria growth medium, and the ability to remove the plasmids from host bacteria by a simple shift in the bacteria growth temperature. These plasmids may also contain an additional nucleotide sequence, GH.sub.3, which confers increased temperature stability.

REFERENCES:
DiJoseph et al., J. Bacteriology 115(1), pp. 399-410 (1973).
Yamamoto et al., J. Bacteriology 146(1), pp. 85-92 (1981).
Kaji et al., "Drug Resistance in Bacteria", Mitsuhashi, Ed., Japan Scientific Press, pp. 59-70 (1982).
Kamio et al., J. Bacteriology 158(1), pp. 307-312 (1984).
Mochida et al., J. Bacteriology 173(8), pp. 2600-2607 (1991).
Kaji et al., Reprint from: The Roots of Modern Biochemistry, Eds., Kleinkauf et al. (1988).
Okawa et al., Biochemical and Biophysical Research Comm., vol. 142, No. 3, pp. 1084-1088 (1987).
Abeles et al., J. Mol. Biol., vol. 173, pp. 307-324 (1984).
Chattoraj et al., Plasmids in Bacteria, Plenum Pub. Co. New York, NY, pp. 355-381 (1985).

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