Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-01-09
2007-01-09
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100
Reexamination Certificate
active
10079043
ABSTRACT:
The invention relates to a method of a mass-spectrometric analysis of known mutation sites in the genome, such as single nucleotide polymorphisms (SNPs), using the method of restricted primer extension. The invention consists of the use of primers with a photocleavable linker. The linker creates a gap in a DNA strand which is almost the same size as a natural DNA building block (nucleoside). The linker forms a bridge over a base pair without inhibiting hybridization or enzymatic extension. However, the linker allows the primers to be cleaved after extension in order to obtain short DNA fragments which can be more easily detected on the mass spectrometer.
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Phillip Ordoukhanian and John-Stephen Taylor, Design and Synthesis of a Versatile Photocleavable DNA Building Block. Application to Phototriggered Hybridization, American Chemical Society 1995, vol. 117, pp. 9570-9571, St. Louis, Missouri.
Sascha Sauer, et al., A novel procedure for efficient genotyping of single nucleotide polymorphisms, Nucleic Acids Research, 2000, vol. 28, No. 5, Oxford University Press, Berlin-Dahlem, Germany.
Fröhlich Thomas
Hausch Felix
Jäschke Andres
Kostrzewa Markus
Wenzel Thomas
Bruker Saxonia Analytik GmbH
Horlick Kenneth R.
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