Mutated Tn5 transposase proteins and the use thereof

Details Mutated Tn5 transposase proteins and the use thereof Mutated Tn5 transposase proteins and the use thereof

2005-08-02

2009-10-27

Hutson, Richard G (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S183000, C435S194000, C435S091520

Reexamination Certificate

active

07608434

ABSTRACT:
Transposase proteins that are modified relative to and have higher transposase activities than the wild-type Tn5 transposase are disclosed. A transposase protein of the present invention differs from the wild-type Tn5 transposase at amino acid position 41, 42, 450, or 454 and has greater avidity than the wild-type Tn5 transposase for at least one of a Tn5 outside end sequence as defined by SEQ ID NO:3, a Tn5 inside end sequence as defined by SEQ ID NO:4, and a modified Tn5 outside end sequence as defined by SEQ ID NO:5. Also disclosed are various systems and methods of using the transposase proteins of the present invention for in vitro or in vivo transposition.

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