Mutated thermostable nucleic acid polymerase enzyme from Thermus

Details Mutated thermostable nucleic acid polymerase enzyme from Thermus Mutated thermostable nucleic acid polymerase enzyme from Thermus

1993-08-27

1995-10-03

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

43525233, 4353201, 435194, 536 232, 935 10, 935 14, C12N 1554, C12N 912

Patent

active

054551706

ABSTRACT:
A purified thermostable enzyme is derived from the eubacterium Thermus species Z05. The enzyme has DNA polymerase, activity reverse transcriptase activity, and optionally 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.

REFERENCES:
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4965188 (1990-10-01), Mullis et al.
Chien et al., 1976, "Deoxyribonucleic Acid Polymerase From the Extreme Thermophile Thermus aquaticus" J. Bacteriology 127(3):1550-1557.
Degryse et al., 1978, "A Comparative Analysis of Extreme Thermophilic Bacteria Belonging to the Genus Thermus" Arch. Microbiol. 117:189-196.
Kaledin et al., 1980, "Isolation and Properties of DNA Polymerase From Extremely Thermophilic Bacterium Thermus aquaticus YT1" 45(4):494-501.
Degryse et al., 1981, "Studies on the Central Metabolism of Thermus aquaticus an Extreme Thermophilic Bacterium Anaplerotic Reactions and Their Regulation" Arch. Microbiol. 129:173-177.
Suggs et al., 1981, "Use of Synthetic Oligonucleotides as Hybridization Probes: Isolation of Cloned cDNA Sequences for Human Beta2-Microglobulin" Proc. Natl. Acad. Sci. USA 78(11):6613-6617.
Young et al., 1983, "Efficient Isolation of Genes by Using Antibody Probes" Proc. Natl. Acad. Sci. USA 80:1194-1198.
Microbiology, 5th Ed., 1986, pp. 41-49, ed. M. J. Pelczal, Jr. et al., published by McGraw-Hill Book Company (New York) "The Characterization, Classification, and Indentification of Microorganisms".
Williams, R. A. D., "Biochemical Taxonomy of the Genus Thermus", published in: Microbiology of Extreme and its Potential for Biotechnology, pp. 82-97, eds., M. S. DaCosta, J. C. Durate and R. A. D. Williams, a volume of The Proceedings of the Federation of European Microbiological Societies Symposium held in Troia, Portugal, 18-23 Sep. 1988, published by Elsevier Applied Science (London and New York).
Bernad et al., 1989, "A Conserved 3'-5' Exonuclease Active Site in Prokaryotic and Eukaryotic DNA Polymerases" Cell 59:219-228.
Gelfand, D. H., PCR Technology, Principles and Applications for DNA Amplification, Chapter 2, Entitled "Taq DNA Polymerase" pp. 17-22 (1989), Ed. by H. A. Erlich.
Laywer et al., 1989, "Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene from Thermus Aquaticus" J. Biol. Chem. 264(11):6427-6437.
Leavitt and Ito, 1989, "T5 DNA Polymerase: Structural-Functional Relationships to Other DNA Polymerases" Proc. Natl. Acad. Sci. USA 86:4465-4469.
Microbiology, 4th Ed., 1990, pp. 11-19, edited by B. D. Davis et al., published by J. B. Lippincott Co. (Philadelphia) "Evolution of Microbiology and of Microbes".

Affiliated with

Also associated with

Rating

Mutated thermostable nucleic acid polymerase enzyme from Thermus does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Mutated thermostable nucleic acid polymerase enzyme from Thermus, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mutated thermostable nucleic acid polymerase enzyme from Thermus will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1076961