Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1995-06-06
1999-02-23
Ketter, James
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
536 231, 536 241, C07K 1300, C07H 2104
Patent
active
058745343
ABSTRACT:
The present invention provides mutant proteins of steroid hormone receptors. These mutant proteins are useful in methods of distinguishing a steroid hormone receptor antagonist from a steroid hormone receptor agonist. The present invention also provides plasmids containing mutated steroid hormone receptor proteins and cells transfected with those plasmids. In addition, the present invention provides methods for determining whether a compound is a steroid hormone receptor antagonist or agonist. Also, the present invention provides methods of determining endogenous ligands for steroid hormone receptors. The invention further provides a molecular switch for regulating expression in gene therapy and methods of employing the molecular switch in humans, animals, transgenic animals and plants.
REFERENCES:
patent: 4736866 (1988-04-01), Leder et al.
patent: 4981784 (1991-01-01), Evans et al.
patent: 5283173 (1994-02-01), Fields et al.
patent: 5364791 (1994-11-01), Vegeto et al.
patent: 5928422 (1994-03-01), Schwartz et al.
Braselmann et al., "A selective transcriptional induction system for mammalian cells based on Gal4-estrogen receptor fusion proteins," Proc. Natl. Acad. Sci USA 90:1657-1661 (1993).
Curiel et al., "Gene Transfer to Respiratory Epithelial Cells via the Receptor-mediated Endocytosis Pathway," Am. J. Respir. Cell. Mol. Biol. 6:247-252 (1992).
Uhlen and Moks, "Gene Fusion for Purpose of Expression: An Introduction," Methods in Enzymology 185:129-143 (1990).
Nagaya et al., "Thryoid Hormone Receptor Mutants That Cause Resistance to Thyroid Hormone," J. Biol. Chem. 267:13014-13019 (1992).
Wagner et al., "Transferrin-polycation-DNA complexes: The effect of polycations on the structure of the complex and DNA delivery to cells," Proc. Natl. Acad. Sci. USA 88:4255-4259 (1991).
Elliston et. al.. Superactive estrogen receptors. J. Biol. Chem. vol. 265(20):11517-11521, Jul. 15, 1990.
Pham et. al.. Antiestrogen can establish nonproductive receptor complexes and alter chromatin structure at target enhancers. PNAS USA vol. 88:3125-3129, Apr. 1991.
Gronemeyer et. al.. Mechanisms of hormone action. J. Steroid Bionchem. Molec. Biol. vol. 41(3-8):217-221, Feb. 13, 1992.
Akerblom et al., "Negative Regulation by Glucocorticoids Through Interference with a cAMP Responsive Enhancer," Science 241:350-353 (1988).
Allan et al., "Hormone and Antihormone Induce Distinct Conformational Changes Which are Central to Steroid Receptor Activation," J. Biol. Chem, 267:19513-19520 (1992).
Allan et al., "Ligand-dependent conformational changes in the progesterone receptor are necessary for events that follow DNA binding," Proc. Natl. Acad. Sci. USA 89:11750-11754 (1992).
Barzel, U., "Estrogens in the Prevention and Treatment of Postmenopausal Osteoporosis: A review," Am. J. Med. 85:847-850 (1988).
Beato, M., "Gene Regulation by Steroid Hormones," Cell 56:335-344 (1989).
Beato, M. "Transcriptional control by nuclear receptors," FASEB J. 5:2044-2051 (1991).
Beekman et al., "Transcriptional Activation by the Estrogen Receptor Requires a Conformational Change in the Ligan Binding Domain," Mol. Endocrinol. 7:1266-1274 (1993).
Berry et al., EMBO J. 9(9):2811-2818 (1990).
Cato et al., "Steroids and Growth Promoting Factors in the Regulation of Expression of Genes and Gene Networks," J. Steroid Biochem. Molec. Biol. 43:63-68 (1992).
Celada et al., "Repression of Major Histocompatibility Complex IA Expression by Glucorticoids: The Glucocorticoid Receptor Inhibits the DNA Binding of the X Box DNA Binding Protein," J. Exp. Med. 177:691-698 (1993).
Chu et al., "Efficiency of Cytoplasmic Delivery by ph-Sensitive Liposomes to the Cells in Culture," Pharmaceutical Research 7:824-834 (1990).
Dahlman-Wright et al., "Interaction of the Glucocorticoid Receptor DNA-binding Domain with DNA as a Dimer Is Mediated by a Short Segment of Five Amino Acids," J. Biol. Chem. 266:3107-3112 (1991).
Daneshgari et al., "Endocrine Therapy of Advanced Carcinoma of the Prostate," Cancer 71:1089-1097 (1993).
Denis et al., "Requirement of hormone for thermal conversion of the glucocorticoid receptor to a DNA-binding state," Nature 333:686-688 (1988).
Denis et al., "The Molybdate-stabilized Nonactivated Glucocorticoid Receptor Contains a Dimer of M.sub.r 90,000 Non-hormone-binding Protein," J. Biol. Chem. 262:11803-11806 (1987).
Diamond et al., "Transcriptional Factor Interactions: Selectors of Positive or Negative Regulation from a Single DNA Element," Science 249:1266-1272 (1990).
Dobson et al., "Mutational Analysis of the Chicken Progesterone Receptor," J. Biol. Chem. 264:4207-4211 (1989).
Dreicer and Wilding, "Steroid Hormone Agonists and Antagonists in the Treatment of Cancer," Cancer Investigation 10:27-41 (1992).
Drouin et al., "Glucocorticoid Receptor Binding to a Specific DNA Sequence is Required for Hormone-Dependent Repression of Pro-Opiomelanocortin Gene Transcription," Molecular and Cellular Biology 9:5305-5314 (1989).
Evans, "The Steriod and Tyroid Hormone Receptor Superfamily," Science 240:889-895 (1988).
Fuller et al., "The steroid receptor superfamily: mechanisms of diversity," FASEB J. 5:3092-3099 (1991).
Gauthier et al., "Functional interference between the Spi-1/Pu.1 oncoprotein and steroid hormone or vitamin receptors," EMBO J. 12:5089-5096 (1993).
Haensler and Szoka, "Synthesis and Characterization of a Trigalactosylated Bisacridine Compound to Target DNA to Hepatocytes," Bioconjugate Chem. 4:85-93 (1993).
Heck et al., "A distinct modulating domain in glucocorticoid receptor monomers in the repression of activity of the transcription factor AP-1," EMBO J. 13:4087-4095 (1994).
Hollenberg and Evans, "Multiple and Cooperative Trans-Activation Domains of the Human Glucocorticoid Receptor," Cell 55:899-906 (1986).
Howard and Distelhorst, "Evidence for Intracellular Association of the Glucocorticoid Receptor with the 90-kDa Heat Shock Protein," J. Biol. Chem. 263:3474-3481 (1988).
Ito et al., "Transformation of Intact Yeast Cells Treated with Alkali Cations," J. Bacteriol. 153:163-168 (1983).
Jonat et al., "Antitumor Promotion and Antiinflammation: Down-Modulation of AP-1 (Fos/Jun) Activity by Glucocorticoid Hormone," Cell 62:1189-1204 (1990).
Kawai et al., "New Procedure for DNA Transfection with Polycation and Dimethyl Sulfoxide," Mol. Cell. Biol. 4:1172-1174 (1984).
Kerppola et al., "Fos is a Preferential Target of Glucocortcoid Receptor Inhibition of AP-1 Activty In Vitro," Moll. Cell. Biol. 13:3782-3791 (1993).
Kutoh et al., "Functional Inteference between the Ubiquitous and Constitutive Octamer Transcription Factor 1 (OTF-1) and the Glucocorticoid Receptor by Direct Protein-Protein Interaction Involving the Homeo Subdomain of OTF-1," Mol. Cell. Biol. 12:4960-4969 (1992).
Lanz and Ruscone, "A Conserved Carboxy-Terminal Subdomain Is Important for Ligand Interpretation and Transactivation by Nuclear Receptors," Endocrinology 135:2183-2195 (1994).
Lebeau et al., "P59, an hsp 90-binding Protein," J. Biol. Chem. 267:4281-4284 (1992).
Legendre and Szoka, "Cyclic amphipathic peptide -DNA complexes mediate high-efficency transfection of adherent mammalian cells," Proc. Natl. Acad. Sci. USA 90;893-897 (1993).
Legendre and Szoka, "Delivery of Plasmid DNA into Mammalian Cell Lines Using pH-Sensitive Liposomes: Comparison with Cationic Liposomes," Pharmaceutical Research 9:1235-1242 (1992).
Lerner et al., "Isolation of Subtilisin Pro-sequence Mutations that Affect Formation of Active Protease by Localized Random Polymerase Chain Reaction Mutagenesis," J. Biol. Chem. 265:20085-20086 (1990).
Liu et al., "Hormone-Independent Repression of Ap-1-Inducible Collagenase Promoter Activity by Glucocortoid Receptors," Mol. Cell. Biol. 15:1005-1013 (1995).
Lucibello et al., "Mutual transrepression of Fos and the glucocortocoid receptor: involvement of a functional domain in Fos which is absent in FosB,," EMBO J. 9:2827-2834 (1990).
Mak et al., "Expression of Functional Chicken Oviduct Progesterone Receptors in Yeast (Saccharomyces cerevisiae)," J. Biol. Chem. 264:21613-21618 (1989).
McDonnell et al., "Reconstitution of the Vitamin D-Responsive O
McDonnell Donald P.
O'Malley Bert W.
Schrader William T.
Tsai Ming-Jer
Vegeto Elisabetta
Baylor College of Medicine
Ketter James
Sandals William
LandOfFree
Mutated steroid hormone receptors, methods for their use and mol does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Mutated steroid hormone receptors, methods for their use and mol, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mutated steroid hormone receptors, methods for their use and mol will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-308103