Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
1998-12-09
2003-07-29
Yucel, Remy (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S235100, C435S325000, C536S023100
Reexamination Certificate
active
06599698
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates generally to the fields of molecular endocrinology and receptor pharmacology. It further relates to molecular switches for gene therapy. More specifically, the present invention relates to a novel in vivo method for the identification of steroid hormone receptor agonists and antagonists and to a molecular switch involving a modified steroid receptor for up-regulating and down-regulating the synthesis of heterologous nucleic acid sequences which have been inserted into cells.
2. Description of the Related Art
Steroid receptors are responsible for the regulation of complex cellular events, including transcription. The ovarian hormones, estrogen and progesterone, are responsible, in part, for the regulation of the complex cellular events associated with differentiation, growth and functioning of female reproductive tissues. These hormones play also important roles in development and progression of malignancies of the reproductive endocrine system.
The biological activity of steroid hormones is mediated directly by a hormone and tissue-specific intracellular receptor. The physiologically inactive form of the receptor may exist as an oligomeric complex with proteins, such as heat-shock protein (hsp) 90, hsp70 and hsp56. Upon binding its cognate ligand, the receptor changes conformation and dissociates from the inhibitory heteroligomeric complex. Subsequent dimerization allows the receptor to bind to specific DNA sites in the regulatory region of target gene promoters. Following binding of the receptor to DNA, the hormone is responsible for mediating a second function that allows the receptor to interact specifically with the transcription apparatus. Displacement of additional inhibitory proteins and DNA-dependent phosphorylation may constitute the final steps in this activation pathway.
Cloning of several members of the steroid receptor superfamily has facilitated the reconstitution of hormone-dependent transcription in heterologous cell systems. Subsequently, in vivo and in vitro studies with mutant and chimeric receptors have demonstrated that steroid hormone receptors are modular proteins organized into structurally and functionally defined domains. A well defined 66 amino acid DNA binding domain (DBD) has been identified and studied in detail, using both genetic and biochemical approaches. The ligand (hormone) binding domain (LBD), located in the carboxyl-terminal half of the receptor, consists of about 300 amino acids. It has not been amenable to detailed site-directed mutagenesis, since this domain appears to fold into a complex tertiary structure, creating a specific hydrophobic pocket which surrounds the effector molecule. This feature creates difficulty in distinguishing among amino acid residues that affect the overall structure of this domain from those involved in a direct contact with the ligand. The LBD also contains sequences responsible for receptor dimerization, hsp interactions and one of the two transactivation sequences of the receptor.
Gene replacement therapy requires the ability to control the level of expression of transfected genes from outside the body. Such a “molecular switch” should allow specificity, selectivity, precision safety and rapid clearance. The steroid receptor family of gene regulatory proteins is an ideal set of such molecules. These proteins are ligand activated transcription factors whose ligands can range from steroids to retinoids, fatty acids, vitamins, thyroid hormones and other presently unidentified small molecules. These compounds bind to receptors and either up-regulate or down-regulate. The compounds are cleared from the body by existing mechanisms and the compounds are non-toxic.
The efficacy of a ligand is a consequence of its interaction with the receptor. This interaction can involve contacts causing the receptor to become active (agonist) or for the receptor to be inactive (antagonist). The affinity of antagonist activated receptors for DNA is similar to that of agonist-bound receptor. Nevertheless, in the presence of the antagonist, the receptor cannot activate transcription efficiently. Thus, both up and down regulation is possible by this pathway.
The present invention shows that receptors can be modified to allow them to bind various ligands whose structure differs dramatically from the naturally occurring ligands. Small C-terminal alternations in amino acid sequence, including truncation, result in altered affinity and altered function of the ligand. By screening receptor mutants, receptors can be customized to respond to ligands which do not activate the host cells own receptors. Thus regulation of a desired transgene can be achieved using a ligand which will bind to and regulate a customized receptor.
Steroid receptors and other mammalian transcription regulators can function in yeast. This fact, coupled with the ease of genetic manipulation of yeast make it a useful system to study the mechanism of steroid hormone action.
A long felt need and desire in this art would be met by the development of methods to identify steroid hormone receptors agonists and antagonists. The development of such a method will facilitate the identification of novel therapeutic pharmaceuticals. Additionally, the present invention provides a novel approach to regulate transcription in gene therapy. By using modified steroid receptors and custom ligands, up-regulation and down-regulation of inserted nucleic acid sequences can be achieved.
SUMMARY OF THE INVENTION
An object of the present invention is a modified steroid hormone receptor protein for distinguishing hormone antagonists and agonists.
An additional object of the present invention is a plasmid containing a modified hormone receptor.
A further object of the present invention are transfected cells containing modified hormone receptors.
Another object of the present invention is a transformed cell containing modified hormone receptors.
An additional object of the present invention is a method for determining agonist activity of a compound for steroid hormone receptors.
A further object of the present invention is a method for determining antagonist activity of a compound for steroid hormone receptors.
An object of the present invention is a method for determining endogenous ligands for steroid hormone receptors.
An object of the present invention is an endogenous ligand for a modified steroid receptor.
An object of the present invention is a molecular switch for regulated expression of a nucleic acid sequence in gene therapy.
An additional object of the present invention is a molecular switch which binds non-natural ligands, anti-hormones and non-native ligands.
A further object of the present invention is a molecular switch comprised of a modified steroid receptor.
An additional object of the present invention is a method for regulating expression of nucleic acid sequence in gene therapy.
A further object of the present invention is a modified progesterone receptor with a native binding domain replaced with GAL-4 DNA.
An additional object of the present invention is to add a more potent activation domain to the receptor.
Another object of the present invention is a method of treating senile dementia or Parkinson's disease.
Thus, in accomplishing the foregoing objects, there is provided in accordance with one aspect of the present invention a mutated steroid hormone receptor protein. This mutated steroid hormone receptor protein is capable of distinguishing a steroid hormone receptor antagonist from a steroid hormone receptor agonist.
In specific embodiments of the present invention, the receptor is selected from a group consisting of estrogen, progesterone, androgen, Vitamin D, COUP-TF, cis-retonic acid, Nurr-1, thyroid hormone, mineralocorticoid, glucocorticoid-&agr;, glucocorticoid-&bgr;, ecdysterone and orphan receptors.
In a preferred embodiment the mutated steroid receptor is mutated by deletion of carboxy terminal amino acids. Deletion usually comprises from one to about 120 amino acids and
McDonnell Donald P.
O'Malley Bert W.
Schrader William T.
Tsai Ming-Jer
Vegeto Elisabetta
Baylor College of Medicine
Perkins Coie LLP
Sandals William
Wise Michael J.
Yucel Remy
LandOfFree
Mutated steroid hormone receptors, methods for their use and... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Mutated steroid hormone receptors, methods for their use and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mutated steroid hormone receptors, methods for their use and... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3057170