Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1992-01-15
1994-12-20
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4352402, 536 241, C12N 1500, C12N 500, C07H 1700
Patent
active
053745442
ABSTRACT:
A mutated skeletal actin promoter which differs from the naturally occurring DNA sequence by nucleotide substitution has been found to increase gene expression. The mutation sites are specific to the cis-acting regions of the gene. Double mutations created a strong promoter. Transformed myogenic cells had ten times the transcriptional activity over the wild type. The mutated skeletal actin promoter can be used to overly express polypeptides, particularly in myogenic cells.
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Chow King L.
Schwartz Robert J.
Baylor College of Medicine
Crouch Deborah
Weimar Elizabeth C.
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