Mutated nonactivating IgG2 domains and anti CD3 antibodies incor

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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C07K 1600

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active

058345973

ABSTRACT:
The invention provides mutated IgG2 constant regions and anti-CD3 antibodies incorporating the same. Such antibodies specifically bind to the CD3 antigen on T-cells but induce reduced mitogenic response compared with otherwise identical antibodies bearing natural IgG2 constant regions. The antibodies can be used for treating disorders requiring immune suppression with fewer side effects than result from treatment with prior anti-CD3 antibodies.

REFERENCES:
patent: 5585089 (1996-12-01), Owen et al.
Anasetti Claudio et al., "Induction of Specific Nonresponsiveness in Unprimed Human T Cells by Anti-CD3 Antibody and Alloantigen," J. Exp. Med., Dec. 1990, vol. 172, pp. 1693-1700.
Anasetti Claudio et al., "Treatment of Acute Graft-Versus-Host Disease With a Nonmitogenic Anti-CD3 Monoclonal Antibody," Translation, Nov. 1992, vol. 54, No. 5, pp. 844-851.
Jolliffe Linda K., "Humanized Antibodies: Enhancing Therapeutic Utility Through Antibody Engineering," Intern. Rev. Immunol., 1993, vol. 10, pp. 241-250.
Alegre, M.L. et al. 1994. Transplantation, 57:1537-1543.
Lurd, J. et al. 1991. J. Immunol. 147:2657-2662.
Pakkeu, PWHI. et al. 1992. J. Immunol. 148:695-701.

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