Mutated .beta.-lactam acylase genes

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound having a 1-thia-4-aza-bicyclo

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435 44, 435 45, 435230, 435228, 4353201, 4352523, 536 232, C12N 984, C12N 1555, C12P 3700

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054570328

ABSTRACT:
New mutant .beta.-lactam acylases are provided exhibiting altered substrate specificities. These .beta.-lactam acylases are obtained by expression of a gene encoding said .beta.-lactam acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type .beta.-lactam acylase.

REFERENCES:
patent: 4774179 (1988-09-01), Ichikawa et al.
patent: 5168048 (1992-12-01), Quax
patent: 5192678 (1993-03-01), Iwami et al.
Schumacher, et al., "Penicillin acylase from E. coli: unique gene--protein relation," Nucleic Acids Research, vol. 14, No. 14 (1986).
Barbero, et al., "Complete nucleotide sequence of the penicillin acylase gene from Kluyvera citrophilia," Gene, vol. 49, pp. 69-80 (1986).
Daumy, "Role of protein subunits in Proteus rettgeri Penicillin G Acylase," J. Bacteriol., vol. 163, No. 3, Sep. 1985 pp. 1279-1281.
Williams, et al., "Penicillin G Acylase (E.C.3.4.1.11) substrate specificity modification by in vitro mutagenesis," Cell Biochem. 9B/supplement (1985) p. 99, No. 656.
Forney, et al. "Selection of amidases with novel substrate specificities from Penicillin Amidase of Escherichia coli," Applied & Environmental Microbiology, vol. 55, No. 10, Oct. 1989 pp. 2550-2555.
Forney, et al., "Alteration of the catalytic efficiency of Penicillin Amidase from Escherichia coli," Applied & Environmental Microbiology, vol. 55, No. 10, pp. 2556-2560 1989.
Matsuda, A., et al. (1987) J. Bacteriol. 169 (12), 5815-5820.
Matsuda A. et al. (1987) J. Bateriol. 169 (12), 5821-5826.
Norrander, J. et al (1983) Gene 26, 101-106.
Stanssens, P. et al. (1989) Nuc. Acids Res. 12(12) 4441-4454.

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