Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2008-10-13
2010-06-08
Pak, Yong D (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S440000, C435S004000, C435S006120, C435S069100, C435S071100, C435S026000, C435S252300, C435S320100, C536S023200
Reexamination Certificate
active
07732179
ABSTRACT:
A mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) is provided with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alamine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from theA. calcoaceticuss-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.
REFERENCES:
patent: 02/34919 (2002-05-01), None
patent: 2006/008132 (2006-01-01), None
Branden et al. Introduction to Protein Structure, Garland Publishing Inc., New York, p. 247, 1991.
“Molecular Engineering of PQQGDH and its Applications,” S. Igarashi et al., Archives of Biochemistry and Biophysics, vol. 428, No. 1, Aug. 1, 2004, pp. 52-63.
Beck Daniela
Boenitz-Dulat Mara
Der Eltz Herbert Von
Kratzsch Peter
Schmuck Rainer
Barnes & Thornburg LLP
Pak Yong D
Roche Diagnostics Operations Inc.
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