Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1996-08-30
1998-06-23
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
4352523, 43525233, 4352541, 4352551, 4353201, 4352561, 536 231, 536 232, 536 237, 536 2374, C12N 902
Patent
active
057704198
ABSTRACT:
The present invention relates to mutants of a blue multi-copper oxidase, comprising (a) a substitution of one or more amino acid residues with other amino acid residues, (b) an insertion of one or more amino acid residues and/or (c) a deletion of one or more amino acid residues, wherein the substitution, insertion or deletion is carried out at a position which is located no greater than 15 .ANG. from a Type I (T1) copper site. The present invention also relates to nucleic acid constructs comprising a nucleic acid sequence encoding the mutants of the present invention, host cells comprising the construct of the present invention, and methods for producing mutants of the present invention.
REFERENCES:
Germann, Ursula A. et al., "Characterization Of Two Allelic Forms of Neurospora Crassa Laccase", 1988 by The American Society For Biochemistry And Molecular Biology, Inc., Jan. 15, vol. 263, No. 2, pp. 885-896.
Kojima Yasushi et al., "Cloning, Sequence Analysis, and Expression of Ligninolytic Phenoloxidase Genes of the White-rot Basidiomycete Coriolus", 1990 by The American Society of Biochemistry And Molecular Biology, vol. 265, No. 25, Sep. 5, pp. 15224-15230.
Askwith, Candice et al., "The FET3 Gene of S. Cerevisiae Encodes A Multicopper Oxidase Required For Ferrous Iron Uptake", Jan. 28 1994 by Cell Press, vol. 76, pp. 403-410.
Messerschmidt et al. "X-ray crystal structure of the Blue oxidase, Ascorbate oxidase from zucchini" J. Mol. Biol. 206, 513-529, 1989.
Messerschmidt et al. "Refined crystal structure of ascorbate oxidase at 1.9 A resolution" J. Mol. Biol. 224, 179-205, 1992.
Xu et al. "A study of a series of recombinant fungal lacacases and bilirubin oxidase that exhibit significant differences in redox potential, substrate specificity and stability" Biochem. Biophys. Acta 1292, 303-311, 1996.
Xu, F. "Oxidation of phenols, anilines, and benzenethiols by fungal laccases: Correlation between activity and redox potentials as well as halide inhibition" Biochemistry 35, 7607-7614, 1996.
Berka Randy M.
Feng Xu
Wahleithner Jill Angela
Harrington James H.
Nashed Nashaat T.
Novo Nordisk Biotech Inc.
Wax Robert A.
Zelson Steve T.
LandOfFree
Mutants of Myceliophthora laccase with enhanced activity does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Mutants of Myceliophthora laccase with enhanced activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mutants of Myceliophthora laccase with enhanced activity will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1393630