Mutants of human antithrombin III

Details Mutants of human antithrombin III Mutants of human antithrombin III

1992-12-18

1997-04-08

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

514 12, 530350, C12N 964, A61K 3817

Patent

active

056187130

ABSTRACT:
Mutants of AT III which have been advantageously modified at one or more potential glycosylation sites or at the Arg393 position are described. As a rule, combination of the mutations enhances the advantageous modifications.

REFERENCES:
patent: 4632981 (1986-12-01), Bock et al.
"Chromogene Substratmethoden zur Antithrombin III-Bestimmung", J. Schrader et al., (1986), Das Arztliche Laboratorium, pp. 111-114.
Zettlmeissl et al., "Influence of Glycosylation on the Functional Properties of Human Therapeutic Plasma Proteins", Protein Glycosylation: Cellular, Biotechnological and Analytical Aspects, GBF Monographs, vol. 15 (ed. H.5. Conradt), pp. 259-268, 1991.
Bowie et al., "Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions", Science, vol. 247, pp. 1306-1310, Mar. 1990.
G. Zettlemeissl et al., "Expression of Biologically Active Human Anti-thrombin III in Chinese Hamster Ovary Cells," Bio/Technology, vol. 5, pp. 720-725 (Jul. 1987).
M. Hoylaerts et al., "Involvement of Heparin Chain Length in the Heparin-catalyzed Inhibition of Thrombin by Antithrombin III," J. Biol. Chem, vol. 259, No. 9, pp. 5670-5677 (May 1984).
C. Peterson et al., "Isolation and Characterization of an Antithrombin III Variant with Reduced Carbohydrate Content and Enhanced Heparin Binding," J. Biol. Chem., vol. 260, No. 1, pp. 610-615 (Jan. 1985).
S. Brennan et al. "Physiological Variant of Antithrombin-III Lacks Carbohydrate Sidechain at Asn 135," FEBS Letters, vol. 219, No. 2, pp. 431-436 (Jul. 1987).
Yasushi Morinaga et al., "Improvement of Oligonucleotide-Directed Site-Specific Mutagenesis Using Double-Stranded Plasmid DNA," Bio/Technology, pp. 636-639 (Jul. 1984).
W. Kramer et al., "The Gapped Duplex DNA Approach to Oligonucleotide-Directed Mutation Construction," Nucl. Acids Res., vol. 12, No. 24, pp. 9441-9456 (1984).
G. Zettlmeissl et al., "Efficient Expression System for Human Antithrombin III in Baby Hamster Kidney Cells," Behring Inst. Mitt., No. 82, pp. 26-34 (1988).
F. Sanger et al., "DNA Sequencing with Chain-Terminating Inhibitors," Proc. Natl. Acad. Sci. USA, vol. 74, No. 12, pp. 5463-5467. (1977).
A. Hensen et al., "III. Antithrombin III Assay," Thromb. Diatl. Haemorrh., vol. 9, pp. 18-29 (1963).
J. Schrader et al., "Methoden zur Bestimmung des Antithrombin III (Methods for the Determination of Antithrombin III)", Arztl. Lab., vol. 29, pp. 35-39, (1983).
S. Engelbrecht et al., "Separation of Human Leucocyte Enzymes Alanine Aminopeptidase, Cathepsin G, Collagenase, Elastase and Myeloperoxidase," Hoppe-Seyler's Z. Physiol. Chem., vol. 363, pp. 305-315, (Mar. 1982).
K. Nakajima et al., "Mapping the Extended Substrate Binding Site of Cathepsin G and Human Leukocyte Elastase," J. Biol. Chem., vol. 254, No. 10, pp. 4027-4031 (May 1979).
Jorgensen et al. Biochem J. 231:59-63 (1985).
Molho-Sabatier et al. J Clin Invest 84:1236-1242 (1989).

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