Mutants of gram negative mucosal bacteria and application...

Details Mutants of gram negative mucosal bacteria and application... Mutants of gram negative mucosal bacteria and application...

2006-03-14

2006-03-14

Smith, Lynette R. F. (Department: 1645)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Bacterium or component thereof or substance produced by said...

C424S178100, C424S184100, C424S200100, C424S234100, C424S250100, C424S278100, C424S282100, C435S041000, C435S243000, C435S235100, C435S252100, C530S388400

Reexamination Certificate

active

07011836

ABSTRACT:
It is possible to inactivate the early stage of lipid A synthesis of mucosal gram negative bacteria without compromising cell viability. In particular the lpxA gene inN. meningitidiswas mutated and resulting lpxA knockout mutants were found to be completely lipopolysaccharide (LPS)-deficient. The major outer membrane proteins (OMPs) were detected in normal amounts. The finding provides important implications for understanding of structure and biogenesis of the outer membrane. On a practical level, the availability of LPS-deficient mutants of pathogenic mucosal bacteria such asN. meningitidisopens up new avenues to vaccine development. It enables easy isolation of endotoxin-free purified proteins, outer membranes or even whole-cell preparations for use in immunisation.

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