Mutants of deoxycytidine kinase having extended enzymatic...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S440000, C536S023100, C536S024300

Reexamination Certificate

active

07547513

ABSTRACT:
The invention relates to a method for artificial in vivo evolution of proteins, said method making it possible to bring about the evolution of a protein X by complementation of a relative protein Y, X and Y both belonging to the same class of enzyme commission (EC) nomenclature or belonging to related classes. The mutants D133E and R104Q of desoxycytidine kinase (DCK) were obtained; both of said mutations result in acquisition of thymidine kinase activity by DCK.

REFERENCES:
patent: 5605793 (1997-02-01), Stemmer
patent: 5811238 (1998-09-01), Stemmer et al.
patent: 5830696 (1998-11-01), Short
patent: 6153745 (2000-11-01), Williams et al.
patent: 6207150 (2001-03-01), Crouzet et al.
patent: 6242222 (2001-06-01), Gifford
patent: 6444682 (2002-09-01), Simmonds et al.
patent: 2002/0028922 (2002-03-01), Glazer et al.
patent: 2002/0083488 (2002-06-01), Miyawaki et al.
patent: 2002/0151019 (2002-10-01), Shanklin
patent: 2002/0182724 (2002-12-01), Zambrowicz et al.
patent: 2003/0049614 (2003-03-01), Hogrefe et al.
patent: 2003/0134351 (2003-07-01), Vega et al.
patent: 2003/0134421 (2003-07-01), Harrington et al.
patent: 2003/0171543 (2003-09-01), Bott et al.
patent: 2003/0175887 (2003-09-01), Short
patent: 2003/0194807 (2003-10-01), Crea
patent: 2003/0207830 (2003-11-01), Deville-Bonne et al.
patent: 2004/0048268 (2004-03-01), Delcourt et al.
patent: 2004/0209367 (2004-10-01), Charles et al.
patent: 2005/0009080 (2005-01-01), Short
patent: 0 939 130 (1999-09-01), None
patent: WO 95/22622 (1995-08-01), None
patent: WO 97/29196 (1997-08-01), None
patent: WO 01/88106 (2001-11-01), None
Spee et al. Efficient random mutagenesis method and adjustable mutation frequency by use of PCR and dITP. Nucleic Acids Research 21 (3) : 777-778 (1993).
Zaccolo et al., An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues. Journal of Molecular Biology 255 (4) : 589-603 (1996).
Zaccolo et al., The effect of high-frequency random mutagenesis on in vitro protein evolution : A study on TEM-1 β-lactamase. Journal of Molecular Biology 285 (2) : 775-783 (1999).
Wang, J. et al., “AnEsherichia coliSystem Expressing Human Deoxyribonucleoside Salvage Enzymes for Evaluation of Potential Antiproliferative Nucleoside Analogs”, Antimicrobial Agents and Chemotheraphy, vol. 42, No. 10, pp. 2620-2625, (Oct. 1998).
Bouzon, M. et al., “Human deoxycytidine kinase as a conditional mutator inEsherichia coli”, Academy of Sciences, vol. 320, No. 6, pp. 427-434, (Jun. 1997).
Okajima, T. et al., “Site-directed mutagenesis of AMP-binding residues in adenylate kinase”, Federation of European Biochemical Societies, vol. 334, No. 1, pp. 86-88, (Nov. 1993).
Chottiner, E. et al., “Cloning and expression of Human deoxycytidine kinase cDNA”, Proceedings of the National Academy of Sciences, vol. 88, pp 1531-1535, (Feb. 1991).
Wang, J. et al., “Expression of Human Mitochondrial Thymidine Kinase inEsherichia coli: Correlation between the Enzymatic Activity of Pyrimidine Nucleoside Analogues and Their Inhibitory Effect on Bacterial Growth”, Biochemical Pharmacology, vol. 59, pp. 1583-1588, Jun. 15, 2000.
Tokunaga, T. et al., “Efficacy of Trimethoprim in Murine Experimental Infection with a Thymidine Kinase-Deficient Mutant ofEsherichia coli”, Antimicrobial Agents and Chemotheraphy, vol. 41, No. 5, pp. 1042-1045, (May 1997).
Kostyuk, D. A. et al., “Mutants of T7 RNA Polymerase That Are Able to Synthesize Both RNA and DNA,”FEBS Letters, 369:165-68 (1995).
Sousa, R. et al., “A Mutant T7 NA Polymerase as a DNA Polymerade,”The EMBO Journal, 14(18):4609-21 (1995).
Wan, L. et al., “In Vitro Evolution of Horse Heart Myoglobin to Increase Peroxidase Activity,”PNAS USA, 95:12825-31 (1998).
“Enzyme Nomenclature” available at http://www.chem.qmul.ac.uk/iubmb/enzyme/index.html.
“Classification and Nomenclature of Enzymes by the Reactions they Catalyse” available at http://ww.chem.qmul.ac.uk/iubmb/enzyme/rules.html.
Bouzon, M. et al., “Human Deoxycytidine Kinase as a Conditional Mutator inEscherichia coli” C.R. Acad. Sci. Paris Life Sciences320:427-34 (1997).
Jewell, N. et al., “Nucleoside Reverse Transcriptase Inhibitors and HIV Mutagenesis”J. Antimicrob. Chemother. 52:547-50 (2003).
Loeb, L. et al., “Lethal Mutagenesis of HIV with Mutagenic Nucleoside Analogs”PNAS-USA96:1492-97 (1999).

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