Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-05-19
2009-06-16
Whisenant, Ethan (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S440000, C536S023100, C536S024300
Reexamination Certificate
active
07547513
ABSTRACT:
The invention relates to a method for artificial in vivo evolution of proteins, said method making it possible to bring about the evolution of a protein X by complementation of a relative protein Y, X and Y both belonging to the same class of enzyme commission (EC) nomenclature or belonging to related classes. The mutants D133E and R104Q of desoxycytidine kinase (DCK) were obtained; both of said mutations result in acquisition of thymidine kinase activity by DCK.
REFERENCES:
patent: 5605793 (1997-02-01), Stemmer
patent: 5811238 (1998-09-01), Stemmer et al.
patent: 5830696 (1998-11-01), Short
patent: 6153745 (2000-11-01), Williams et al.
patent: 6207150 (2001-03-01), Crouzet et al.
patent: 6242222 (2001-06-01), Gifford
patent: 6444682 (2002-09-01), Simmonds et al.
patent: 2002/0028922 (2002-03-01), Glazer et al.
patent: 2002/0083488 (2002-06-01), Miyawaki et al.
patent: 2002/0151019 (2002-10-01), Shanklin
patent: 2002/0182724 (2002-12-01), Zambrowicz et al.
patent: 2003/0049614 (2003-03-01), Hogrefe et al.
patent: 2003/0134351 (2003-07-01), Vega et al.
patent: 2003/0134421 (2003-07-01), Harrington et al.
patent: 2003/0171543 (2003-09-01), Bott et al.
patent: 2003/0175887 (2003-09-01), Short
patent: 2003/0194807 (2003-10-01), Crea
patent: 2003/0207830 (2003-11-01), Deville-Bonne et al.
patent: 2004/0048268 (2004-03-01), Delcourt et al.
patent: 2004/0209367 (2004-10-01), Charles et al.
patent: 2005/0009080 (2005-01-01), Short
patent: 0 939 130 (1999-09-01), None
patent: WO 95/22622 (1995-08-01), None
patent: WO 97/29196 (1997-08-01), None
patent: WO 01/88106 (2001-11-01), None
Spee et al. Efficient random mutagenesis method and adjustable mutation frequency by use of PCR and dITP. Nucleic Acids Research 21 (3) : 777-778 (1993).
Zaccolo et al., An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues. Journal of Molecular Biology 255 (4) : 589-603 (1996).
Zaccolo et al., The effect of high-frequency random mutagenesis on in vitro protein evolution : A study on TEM-1 β-lactamase. Journal of Molecular Biology 285 (2) : 775-783 (1999).
Wang, J. et al., “AnEsherichia coliSystem Expressing Human Deoxyribonucleoside Salvage Enzymes for Evaluation of Potential Antiproliferative Nucleoside Analogs”, Antimicrobial Agents and Chemotheraphy, vol. 42, No. 10, pp. 2620-2625, (Oct. 1998).
Bouzon, M. et al., “Human deoxycytidine kinase as a conditional mutator inEsherichia coli”, Academy of Sciences, vol. 320, No. 6, pp. 427-434, (Jun. 1997).
Okajima, T. et al., “Site-directed mutagenesis of AMP-binding residues in adenylate kinase”, Federation of European Biochemical Societies, vol. 334, No. 1, pp. 86-88, (Nov. 1993).
Chottiner, E. et al., “Cloning and expression of Human deoxycytidine kinase cDNA”, Proceedings of the National Academy of Sciences, vol. 88, pp 1531-1535, (Feb. 1991).
Wang, J. et al., “Expression of Human Mitochondrial Thymidine Kinase inEsherichia coli: Correlation between the Enzymatic Activity of Pyrimidine Nucleoside Analogues and Their Inhibitory Effect on Bacterial Growth”, Biochemical Pharmacology, vol. 59, pp. 1583-1588, Jun. 15, 2000.
Tokunaga, T. et al., “Efficacy of Trimethoprim in Murine Experimental Infection with a Thymidine Kinase-Deficient Mutant ofEsherichia coli”, Antimicrobial Agents and Chemotheraphy, vol. 41, No. 5, pp. 1042-1045, (May 1997).
Kostyuk, D. A. et al., “Mutants of T7 RNA Polymerase That Are Able to Synthesize Both RNA and DNA,”FEBS Letters, 369:165-68 (1995).
Sousa, R. et al., “A Mutant T7 NA Polymerase as a DNA Polymerade,”The EMBO Journal, 14(18):4609-21 (1995).
Wan, L. et al., “In Vitro Evolution of Horse Heart Myoglobin to Increase Peroxidase Activity,”PNAS USA, 95:12825-31 (1998).
“Enzyme Nomenclature” available at http://www.chem.qmul.ac.uk/iubmb/enzyme/index.html.
“Classification and Nomenclature of Enzymes by the Reactions they Catalyse” available at http://ww.chem.qmul.ac.uk/iubmb/enzyme/rules.html.
Bouzon, M. et al., “Human Deoxycytidine Kinase as a Conditional Mutator inEscherichia coli” C.R. Acad. Sci. Paris Life Sciences320:427-34 (1997).
Jewell, N. et al., “Nucleoside Reverse Transcriptase Inhibitors and HIV Mutagenesis”J. Antimicrob. Chemother. 52:547-50 (2003).
Loeb, L. et al., “Lethal Mutagenesis of HIV with Mutagenic Nucleoside Analogs”PNAS-USA96:1492-97 (1999).
Bouzon Madeleine
Marliere Philippe
Pochet Sylvie
Centre National de la Recherche Scientifique "CNRS"
Finnegan Henderson Farabow Garrett & Dunner L.L.P.
Institut Pasteur
Whisenant Ethan
LandOfFree
Mutants of deoxycytidine kinase having extended enzymatic... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Mutants of deoxycytidine kinase having extended enzymatic..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mutants of deoxycytidine kinase having extended enzymatic... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4144992