Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1996-01-16
1998-08-18
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435189, 435 691, 435138, 4351721, 4351723, 435843, 536 232, C12N 904, C12N 902, C12N 1500, C12P 760
Patent
active
057957614
ABSTRACT:
Mutants of 2,5-diketo-D-gluconic acid reductase A an enzyme used to produce 2-keto-L-gluconic acid, a precursor of ascorbic acid (vitamin C), are prepared by site-directed mutagenesis. These mutants may exhibit one or more of the following characteristics: improved temperature stability, increased resistance to substrate inhibition, increased turnover of the substrate by the enzyme and increased affinity for the substrate.
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Anderson Stephen
Powers David B.
Auerbach Jeffrey I.
McCabe Kevin W.
Rutgers The State University of New Jersey
Slobodyansky Elizabeth
Wax Robert A.
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