Mutant urease and method of use for determination of urea

Details Mutant urease and method of use for determination of urea Mutant urease and method of use for determination of urea

1996-07-26

1998-12-08

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving urea or urease

435128, 435227, 435 41, C12Q 158, C12P 1302, C12N 978

Patent

active

058467521

ABSTRACT:
A mutant urease of Klebsiella aerogenes characterized as .alpha.H219Q having a glutamine (Q) in position 219 in place of histidine (H). The resulting enzyme has a low affinity for substrate (high value of K.sub.m) and is particularly useful in assays and test kits for measuring urea concentrations in body fluids.

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