Mutant tyrosine repressor proteins

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C530S825000

Reexamination Certificate

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06933365

ABSTRACT:
A gene coding for a mutant tyrosine repressor having increased positive regulatory activity for expression of the tyrosine phenol lyase gene compared with a tyrosine repressor not having mutation is obtained by introducing a mutation into a tyrosine repressor gene ofErwinia herbicola, introducing the gene into which the mutation is introduced intoEscherichia coliexpressing a lactose operon under the control of a promoter and enhancer of the tyrosine phenol lyase gene derived fromErwinia herbicola, and selecting a transformed strain having increased β-galactosidase activity.

REFERENCES:
patent: 6146878 (2000-11-01), Kumagai et al.
patent: 11-313672 (1999-11-01), None
Cui et al. A mutational analysis of the structural basis for transcriptional activation and monomer-monomer interaction in the TyrR system ofEscherichia coliK-12. J. Bacteriol. (1993) 175(6): 1777-1784.
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