Mutant tyrosine repressor, a gene encoding the same, and a...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S041000

Reexamination Certificate

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07964709

ABSTRACT:
A mutant tyrosine repressor that does not require tyrosine to induce expression of tyrosine phenol-lyase gene is obtained by introducing a mutation into a tyrosine repressor. A microorganism which is able to express large amounts of tyrosine phenol-lyase is obtained by introducing the mutant tyrosine repressor into the microorganism. The microorganism is useful for producing L-DOPA.

REFERENCES:
patent: 6146878 (2000-11-01), Kumagai et al.
patent: 2001-238678 (2001-09-01), None
Definition of revertant (last viewed on Feb. 23, 2010).
NCBI Taxonomy Brower—Pantoea agglomerans (last viewed on Feb. 23, 2010).
Hybridization condition to % identity (last viewed on Feb. 24, 2010).
GC content calculation (last viewed on Feb. 24, 2010).
Kumagai, H., et al., “High-level expression of tyrosine phenol-lyase by mutation of the transcriptional regulator TyrR,” Vitamin B Research Committee, The 376thConference, Abstract of Research Reports, 2000, p. 386, with English translation thereof.
Notice of Reason for Rejection for Japanese Patent App. No. 2004-095147 (Nov. 17, 2009) with English translation thereof.
Cornish, E. C., et al., “Structure of theEscherichia coliK12 Regulatory genetyrR,” J. Biol. Chem. 1986;261(1):403-410.
Katayama, T., et al., “Cloning and Random Mutagenesis of theErwinia herbicola tyrRGene for High-Level Expression of Tyrosine Phenol-Lyase,” App. Environmen. Microbiol. 2000;66(11):4764-4771.
Koyanagi, T., et al., “Effective production of 3,4-dihydroxyphenyl-L-alanine (L-DOPA) withErwinia herbicolacells carrying a mutant transcriptional regulator TyrR,” J. Biotechnol. 2005;115:303-306.
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