Mutant type subtilisin YaB and its application

Details Mutant type subtilisin YaB and its application Mutant type subtilisin YaB and its application

1998-08-18

1999-10-26

Achutamurthy, Ponnathapura

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 691, 435220, 43525231, 4353201, 435471, 435267, 536 232, C12N 954, C12N 1557, C12N 1575, C12S 300

Patent

active

059726830

ABSTRACT:
The invention provides a group of subtilisin YaB mutants, which are obtained by the substitution of the glycine residues at the 124, 151 and 159 positions of wild type subtilisin YaB with other amino acid residues by site directed mutagenesis. The subtilisin YaB mutants have special substrate specificity and relatively higher elastin/casein hydrolyzing activity. The subtilisin YaB mutants can be used in various aspects such as the quality improvement of meat and the protein processing for foodstuff and feedstuff. Moreover, the invention also includes the nucleic acid sequences encoding such enzyme mutants and the uses of such enzyme mutants.

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