Mutant strains of Pseudomonas fluorescens and variants...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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C435S101000

Reexamination Certificate

active

07553656

ABSTRACT:
It is described biologically pure bacterial cultures of mutant strains ofPseudomonas fluorescens, which produces large amounts of alginate. The alginate may contain a certain determined content of mannuronate and guluronate residues, possible presence and determined level of acetyl groups in the alginate, and a desired molecular weight of the alginate. Also high yielding mutants with regulation of alginate production, is described. The invention further provides methods for producing new mutant strains ofPseudomonas fluorescensand variants thereof, and use the resulting strains in alginate production.

REFERENCES:
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patent: 2004/0014197 (2004-01-01), Huisman et al.
patent: WO 94/09124 (1994-04-01), None
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Gimmestad Martin et al., “ThePseudomonas fluorescensalgG protein, but not its mannuronan C-5-epimerase activity, is needed for alginate polymer formation,”Journal of Bacteriology, vol. 185, No. 12, 2003, pp. 3515-3523.
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