Mutant strain of Pichia pastoris which utilizes methanol in the

Details Mutant strain of Pichia pastoris which utilizes methanol in the Mutant strain of Pichia pastoris which utilizes methanol in the

1994-01-13

1997-07-01

Ketter, James S.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

536 241, C12N 115, C12N 1511

Patent

active

056437927

ABSTRACT:
A methylotrophic and glucotrophic mutant strain capable of producing a heterologous protein and a method for producing a heterologous protein, comprising culture of the mutant strain. The mutant strain of the present invention can be grown in a medium containing both methanol and glucose, with the effect that the growth of the strain and production of a heterologous protein proceed at the same time. Accordingly, a heterologous protein can be produced in a large amount in a short time.

REFERENCES:
Waleh et al., Gene, 117: 7-14 (1992).
Warnes et al., Biotech & Bioengineering, 38:1050-1058 (1991).
Titorenko et al., Genetika, 27(5):791-800 (1991).
Database Biotech Abstract Neale et al., Appl. Mol. Genet. Fungi, 1991, pp. 118-128.
Abstract Pap. Am. Chem. Soc., Jain et al., vol. 194th, 30 Aug. 1987, No. 34.
Database Biotech. Abstr., Janowicz et al., Eur. Conf. Biotechnol., pp. 82-83.
Sakai et al., App. Environ. Microbiol., vol. 53, No. 8, 1987, pp. 1812-1818 .

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