Mutant Staphylococcus aureus V8 proteases

Details Mutant Staphylococcus aureus V8 proteases Mutant Staphylococcus aureus V8 proteases

1996-06-03

1998-05-05

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4351721, 4352523, 4353201, 536 232, C12N 952, C12N 1500, C12N 120, C07H 2104

Patent

active

057473218

ABSTRACT:
Mutant proteases are obtained with one or more mutation sites in the natural V8 protease protein, and with enzyme activities even in the presence of high urea concentrations.
Inactivation of enzyme activity is minimized even in the presence of high concentrations of urea, to thus allow lower amounts of enzyme to be added to urea-containing reaction systems and shorten reaction times. As an additional advantage, the ability to cleave proteins in the presence of high urea concentrations makes it possible to obtain hitherto unobtainable peptide fragments.

REFERENCES:
patent: 5532343 (1996-07-01), Bayne et al.
patent: 5536655 (1996-07-01), Thomas et al.
Jean Houmard et al., Staphylococcal Protease: A Proteolytic Enzyme Specific for Glutamoyl Bonds, Dec. 1992, Proc. Nat. Acad. Sci., USA, vol. 69, No. 12, pp. 3506-3509.
Cynthia Carmona et al., Nucleotide Sequence for the Serine Protease Gene of Staphylococcus aureus, 1987, Strain V8, Nucleic Acids Research, vol. 15, No. 16, 6757.
Kazumasa Yoshikawa et al., Recombinant Human Glucagon: Large-Scale Purification and Biochemical Characterization, 1992, Journal of Protein Chemistry, vol. 11, No. 5.
Radheshyam K. Jayaswal et al., Cloning and Expression of a Staphylococcus aureus Gene Encoding a Peptidoglycan Hydrolase Activity, Oct. 1990, Journal of Bacteriology, vol. 172, No. 10, pp. 5783-5788.
M. Yabuta et al., High Expression of a Recombinant Human Calcitonin Precursor Peptide in Escherichia coli, 1995, Appl Microbiol Biotechnol, 42:703-708.
Jeffrey Vieira et al., The pUC Plasmids, An M13mp7-Derived System For Insertion Mutagenesis And Sequencing With Synthetic Universal Primers, 1982, Gene 19:259-268.
Yabuta et al.(1995) J.Fermentation and Bioengineering, 80, 237-243.

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