Mutant Smg p21 protein with GTP binding activity

Details Mutant Smg p21 protein with GTP binding activity Mutant Smg p21 protein with GTP binding activity

1992-09-21

1995-01-03

Draper, Garnette D.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

435 691, 435 694, 4351721, 530399, C07K 1300, C12N 1500

Patent

active

053788109

ABSTRACT:
The present invention provides a GTP binding protein containing the following amino acid sequence, with a molecular weight of about 22K dalton and having GTP binding activity which is inhibited by N-ethyl-maleimide and GTP hydrolyzing activity:
Thr-Ile-Glu-Asp-Ser-Tyr, and a method for the production of a GTP binding protein, which comprises introducing a DNA fragment containing DNA that encodes the GTP binding protein into a cloning site present at the downstream to a promoter of an expression vector, then introducing the expression vector thus constructed into a host, culturing said host, thereby expressing and accumulating the GTP binding protein and then collecting thereof.

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The Journal of Biological Chemistry, vol. 263, No. 23, 1988, pp. 11071-11074, Y. Matsui, et al.
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Biochemical and Biophysical Research Communications, vol. 157, No. 3, 1988, pp. 851-860, M. Hoshijima, et al.
Jackson B. Gibbs, et al. TIBS Sep. 1985, pp. 350-353.

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