Mutant serine acetyltransferase

Details Mutant serine acetyltransferase Mutant serine acetyltransferase

2007-12-25

2007-12-25

Woitach, Joseph (Department: 1633)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S113000, C435S252100, C435S471000, C536S023100

Reexamination Certificate

active

10892112

ABSTRACT:
O-acetylserine, L-cysteine and sulphurous compounds derived therefrom may be produced using a bacterium belonging to the genusEscherichiawhich harbors a mutant feedback-resistant serine acetyltransferases in which the amino acid sequence corresponding to positions from 89 to 96 in a wild-type serine acetyltransferase is replaced with any one of the amino acid sequences shown in SEQ ID NOS: 4 to 9, and feedback inhibition by L-cysteine in the bacterium is desensitized.

REFERENCES:
patent: 4278765 (1981-07-01), Debabov et al.
patent: 5661012 (1997-08-01), Sano et al.
patent: 6040160 (2000-03-01), Kojima et al.
patent: 6218168 (2001-04-01), Leinfelder et al.
patent: 6297031 (2001-10-01), Debabov et al.
patent: 6653111 (2003-11-01), Debabov et al.
patent: 2003/0148473 (2003-08-01), Livshits et al.
patent: 56-018596 (1981-02-01), None
patent: 11-155571 (1999-06-01), None
patent: 11-299491 (1999-11-01), None
Inoue et al., Eur J Biochem, 1999, 266: 220-227.
Murillo et al., Cell Mol Biol Res, 1995, 41: 425-433, Abstract.
Mino, K., et al., “Purification and Characterization of Serine Acetyltransferase fromEscherichia coliPartially Truncated at theC-Terminal Region,” Biosci. Biotechnol. Biochem. 1999; 63(1):168-179.
International Search Report for PCT Appl. No. PCT/JP2004/010216 (Aug. 31, 2004).
Denk D., et al., “L-Cysteine Biosynthesis inEscherichia coli: Nucleotide Sequence and Expression of the Serine Acetyltransferase (cysE) Gene from the Wild-type and a Cysteine-excreting Mutant”, Journal of General Microbiology, vol. 133, pp. 515-525, (1987).
Nakamori S., et al., “Overproduction of L-Cysteine and L-Cystine byEscherichia coliStrains with a Genetically Altered Serine Acetyltransferase”, Applied and Environmental Microbiology, vol. 64, No. 5, pp. 1607-1611 (1998).
Takagi H., et al., “PCR random mutagenesis intoEscherichia coliserine acetyltransferase: isolation of the mutant enzymes that cause overproduction of L-cysteine and L-cystine due to the desensitization to feedback inhibition”, FEBS Letters, vol. 452, pp. 323-327 (1999).
Mino, K., et al., “Increase in the Stability of Serine Acetyltransferase fromEscherihichia coliagainst Cold Inactivation and Proteolysis by Forming a Bienzyme Complex”, Biosci. Biotechnol. Biochem., vol. 65, No. 4, pp. 865-874 (2001).
Neidhardt F. C., et al., “Escherichia coliandSalmonella; Cellular and Molecullar Biology; Second Edition”, ASM PRESS, American Society for Microbiology, 1325 Massachusetts Ave., Washington, D.C., pp. 514-527 (1996).
International Preliminary Report on Patentability for PCT Appl. No. PCT/JP2004/010216 (Jun. 1, 2006).

Affiliated with

Also associated with

Rating

Mutant serine acetyltransferase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Mutant serine acetyltransferase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mutant serine acetyltransferase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3839309