Mutant resistant to cell membrane synthesis inhibitor and proces

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4352525, C12N 1500

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active

050791540

ABSTRACT:
A mutant of an extracellular enzyme-producing microorganism belonging to genus Bacillus, which is resistant to a cell membrane synthesis inhibitor, including vancomycin and ristocetin, is disclosed. The cell membrane synthesis inhibitor-resistant mutant can produce such an enzyme as cellulase, protease, or amylase about 2 to 4 times of the parent strain. Such a mutant can be produced by subjecting an extracellular enzyme-producing microorganism tot a mutation treatment, and culturing said microorganism in a culture medium containing a cell membrane synthesis inhibitor.

REFERENCES:
Bergey's Manual of Systematic Bacteriology, vol. 2, Sneath et al. (ed.), Williams & Wilkins, Baltimore, 1986, pp. 1104-1129.
J. Gen. Appl. Microbiol., vol. 31, No. 4, 1985, pp. 323-328; B. Maruo et al.: "Stepwise enhancement of productivity of thermostable amylase in Bacillus licheniformis by a series of mutation".
Agric. Biol. Chem., vol. 43, No. 11, 1979, pp. 2343-2349; K. Hitotsuyanagi et al.: "Stepwise introduction of regulatory genes stimulating production of alpha-amylase into Bacillus substillis: construction of an alpha-amylase extrahyper producing strain".
Biochemical and Biophysical Research Communications, vol. 70, No. 1, 1976, pp. 125-131, Academic Press, Inc., T. Sasaki et al.: "Hyperproductivity of extracellular alpha-amylase by a tunicamycin resistant mutant of Bacillus subtilis".
Chemical Abstract, vol. 93, No. 7, Aug. 1980, p. 735, Abstract No. 68681y, Columbus, Ohio, US; & JP-A-80 48 387 (B. Maruo et al.) 07-04-1980.
Chemical Abstracts, vol. 93, No. 7, Aug. 1980, p. 735, Abstract No. 686846 Columbus, Ohio, US; & JP-A-80 34 047 (B. Maruo) 10-03-1980.
Chemical Abstracts, vol. 97, No. 7, Aug. 1982, p. 496, Abstract No. 54008e Columbus, Ohio, US; & JP-A-82 47 479 (J. Utaka) 18-03-1982.
Purification and Properties of a Cellulase from Alkalophilic Bacillus sp. No. 1139 by Fumiyasu Fukumori, et al. Journal of General Microbiology (1985), 131, 3339-3345.

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