Mutant purine nucleoside phosphorylase proteins and cellular...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...

Reexamination Certificate

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C435S194000, C435S252300, C435S320100, C536S023200, C514S04400A

Reexamination Certificate

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07488598

ABSTRACT:
A host cell stably transformed or transfected by a vector including a DNA sequence encoding for mutant purine nucleoside cleavage enzymes is provided. The transformed or transfected host cell can be used in combination with a purine substrate to treat tumor cells and/or virally infected cells. A nucleotide sequence encoding mutantE. coliderived purine nucleoside phosphorylase proteins which can be used in conjunction with an appropriate substrate to produce toxins which impair abnormal cell growth is also provided. A method is detailed for the delivery of toxin by generation within target cells or by administration and delivery to the cells from without. Novel purine nucleosides are detailed that yield a cytotoxic purine upon enzymatic cleavage. A synthetic process for nucleosides is also detailed.

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