Mutant pseudorabies virus, and vaccines containing the same

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Recombinant virus encoding one or more heterologous proteins...

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4352351, 4351723, 435236, 4242051, 4242291, A61K 39245, A61K 39295, C12N 701, C12N 704

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056957653

ABSTRACT:
The invention relates to a pseudorabies virus (PRV) not found in nature and having a genome containing a mutation in the protein kinase gene region and/or in the 28K gene region. The mutation is e.g. a nucleic acid substitution, deletion, insertion or inversion, or a combination thereof. The mutations are introduced, by genetic engineering techniques, into the genome of a naturally occuring PRV, preferably NIA-3. Insertions may be nucleic acid sequences encoding antigenic polypeptides characteristic of a pathogen found in pigs. The invention also relates to vaccines containing a pseudorabies virus according to the invention, and to a process for preparing such vaccines.

REFERENCES:
Van Zijl et al. J. Virol. 62(6):2191-95 1988.
Thomsen, D.R. et al. Gene, vol. 57, pp. 261-265, 1987.
"Deletions in the Genomes of Pseudorabies Virus Vaccine Strains and Existence of Four Isomers of the Genomes", Journal of Virology, vol. 49, No. 3, Mar. 1984, pp. 970-979, copyright 1984, by American Society for Microbiology.
"Pseudorabies Virus Avirulent Strains Fail to Express a Major Glycoprotein", Journal of Virology, vol. 56, No. 1, Oct. 1985, pp. 307-311, copyright 1985 by American Society for Microbiology.
"Herpes Simplex Virus 1 Protein Kinase Is Encoded by Open Reading Frame US3 Which Is Not Essential for Virus Growth in Cell Culture" Journal of Virology, vol. 61, No. 5, Sep. 1987, pp. 2896-2901, copyright 1987 by American Society for Microbiology.
"Construction and Characterization of Deletion Mutants of Pseudorabies Virus: a New Generation of Live Vaccines", J. gen. Virol, vol. 68, 1987, pp. 523-534, by W. Quint et al.
"Deletions in Vaccine Strains of Pseudorabies Virus and Their Effect on Synthesis of Glycoprotein gp63", Journal of Virology, vol. 60, No. 3, Dec. 1986, pp. 1166-1169, copyright 1986, by American Society for Microbiology.

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