Mutant proteinase with reduced self-cleavage activity and...

Details Mutant proteinase with reduced self-cleavage activity and... Mutant proteinase with reduced self-cleavage activity and...

2001-06-11

2009-02-24

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S320100, C435S252300, C435S219000, C435S325000, C536S023100

Reexamination Certificate

active

07494786

ABSTRACT:
The present invention provides a mutant 27 kDa NIa proteinase having reduce self-cleavage activity relative to the self-cleavage activity of its wild-type proteinase. The mutant the same substrate cleavage activity as the wild-type proteinase but is more stable than the wild-type proteinase. The present invention also provides a method of obtaining large quantities of active 27 kDa NIa proteinase for use as a tool for purification of other proteins.

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