Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Reexamination Certificate
2003-05-09
2008-11-25
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
C435S183000, C435S135000, C435S069100, C528S274000, C536S023200
Reexamination Certificate
active
07456002
ABSTRACT:
Biodegradable polyester with desired physical properties.A mutant poly(3-hydroxyalkanoic acid) synthase comprising an amino acid sequence of (a) or (b) below.(a) A mutant amino acid sequence derived from SEQ ID No. 1, wherein at least one amino acid selected from the group consisting of glutamic acid at position 130, serine at position 325, serine at position 477 and glutamine at position 481 is substituted by another amino acid.(b) A mutant amino acid sequence derived from SEQ ID No. 1 wherein at least one amino acid selected from the group consisting of glutamic acid at position 130, serine at position 325, serine at position 477 and glutamine at position 481 is substituted by another amino acid, wherein at least one amino acid other than the amino acids at positions 130, 325, 477 and 481 is deleted, substituted and/or added, wherein the mutant amino acid sequence has the activity to biosynthesize biodegradable polyester.
REFERENCES:
patent: 1 120 461 (2001-08-01), None
Hiromi Matsusaki et al., Journal of Bacteriology, Dec. 1998, vol. 180, No. 24, pp. 6459 to 6467.
A.A. Amara et al., Appl. Microbiol. Biotechnol., 2002, vol. 59, pp. 477 to 482.
Yong Jia et al., Biochemistry 2001, vol. 40, pp. 1011 to 1019.
Bernd H.A. Rehm et al., Biochimica et Biophysica Acta 2002, vol. 1594, pp. 178 to 190.
Taguchi et al., Polymer Preprints of the Society of Polymer Science, Japan, vol. 51, No. 1, p. 950, (May 29-31, 2002) (and English Translation).
Doi Yoshiharu
Taguchi Seiichi
Takase Kazuma
Birch & Stewart Kolasch & Birch, LLP
Chowdhury Iqbal H
Prouty Rebecca E.
Riken
LandOfFree
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