Mutant phosphoenolpyruvate carboxylase, its gene, and production

Details Mutant phosphoenolpyruvate carboxylase, its gene, and production Mutant phosphoenolpyruvate carboxylase, its gene, and production

1997-11-10

1999-07-06

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4352523, 43525232, 4353201, 536 231, 536 232, 536 237, C07H 2104, C12N 120

Patent

active

059196947

ABSTRACT:
A phosphoenolpyruvate carboxylase gene, which has mutation such as mutation to replace 625th glutamic acid from the N-terminus of phosphoenolpyruvate carboxylase with lysine, mutation to replace 438th arginine from the N-terminus with cysteine and the like, is introduced into Escherichia coli or coryneform bacteria, so as to produce a phosphoenolpyruvate carboxylase which is not substantially inhibited by aspartic acid, thereby amino acid is efficiently produced.

REFERENCES:
patent: 5616480 (1997-04-01), Sugimoto et al.
Biochem. Biophys. Res. Commun., vol. 45, No. 3, Nov. 5, 1971, pp. 689-694.
J. Biochem., vol. 81, No. 5, 1977, pp. 1473-1485.
J. Biochem., vol. 85, No. 2, Feb. 1979, pp. 423-432.
J. Biochem., vol. 84, No. 4, 1978, pp. 795-803.
Fujita et al. "The primary structure of phosphoenolpyruvate carboxylase of Escherichia coli. Nucleotide sequence . . . " J. Biochem. 95, 1984, pp. 909-916.

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