Mutant of the Erythrina caffra type inhibitor and the use of the

Details Mutant of the Erythrina caffra type inhibitor and the use of the Mutant of the Erythrina caffra type inhibitor and the use of the

1997-10-03

1999-10-26

Sisson, Bradley L.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

530344, 530370, 530379, C07K 100, A61K 3800, A61K 3578

Patent

active

059731184

ABSTRACT:
A polypeptide which has the activity of an inhibitor DE-3 from Erythrina caffra and which reversibly and selectively binds serine proteases from a protein mixture is obtainable by culturing prokaryotic or eukaryotic host cells which have been transformed or transfected with a nucleic acid that codes for the said polypeptide in a manner that allows the host cells to express the said polypeptide under suitable nutrient conditions and isolating the said polypeptide, wherein the polypeptide has an amino acid sequence which is functionally analogous to SEQ ID NO:2, has a partial region that is more than 85% homologous to the region of amino acids 39-139 of this sequence, has two disulfide bridges and begins N-terminally with SEQ ID NO:4 or with a SEQ ID NO:4 extended N-terminally by methionine and has a binding capacity for tissue plasminogen activators of 1.25 MU/ml and more and is particularly suitable for purifying plasminogen activators such as tissue plasminogen activators (t-PA and derivatives).

REFERENCES:
Teixeria et al., Biochimica et Biophysia Acta, vol. 1217, (1994) pp. 23-28, "Site-directed mutagenesis of the synthetic Erythrina trypsin/tissue plasminogen activator (tPA) inhibitor . . . ".

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