Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1995-06-02
1997-08-05
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435134, 435198, 4352541, 43525411, C12P 762, C12N 114, C12N 920
Patent
active
056541813
ABSTRACT:
A mutant fungal strain, namely G. candidum NRRL Y-552, mutant known as Henkel 9-2-3-9 produces a lipase called "UNLipase" providing a selectivity of 25:1 for oleic acid over palmitic acid by the assay procedure employed. UNLipase has a temperature range of operation of between 0 and 40 degrees Celsius (and no activity over 55 degrees Celsius). The optimum pH ranges are between 7.5 and 8.5. Magnesium cations increase activity, whereas calcium cations are inhibitory. The molecular weight of the protein appears to be 65 kDa by size exclusion chromatography. UNLipase shows a high degree of selectivity for hydrolysis, esterification and transesterification.
REFERENCES:
patent: 5470741 (1995-11-01), Oester et al.
Hall Allen L.
Oester Dean A.
Vesper Stephen J.
Drach John E.
Henkel Corporation
Jaeschke Wayne C.
Prats Francisco C.
Szoke Ernest G.
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