Mutant of geotrichum candidum which produces novel enzyme system

Details Mutant of geotrichum candidum which produces novel enzyme system Mutant of geotrichum candidum which produces novel enzyme system

1995-08-09

1998-03-10

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435198, 4352541, 43525411, C12P 764

Patent

active

057260486

ABSTRACT:
A mutant fungal strain, namely G. candidum NRRL Y-552, mutant known as Henkel 9-2-3-9 produces a lipase called "UNLipase" providing a selectivity of 25:1 for oleic acid over palmitic acid by the assay procedure employed. UNLipase has a temperature range of operation of between 0 and 40 degrees Celsius (and no activity over 55 degrees Celsius). The optimum pH ranges are between 7.5 and 8.5. Magnesium cations increase activity, whereas calcium cations are inhibitory. The molecular weight of the protein appears to be 65 kDa by size exclusion chromatography. UNLipase shows a high degree of selectivity for hydrolysis, esterification and transesterification.

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