Mutant of bacterium Clostridium histolyticum, a process for the

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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435220, 435183, 435842, C12N 120, C12N 900, C12N 952

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active

052524817

ABSTRACT:
A mutant of bacterium Clostridium histolyticum, designated Clostridum histolyticum K26 clo 88 of the following characteristics: the lack of a motility system which is a fundamental characteristic of the parenteral strain; and, the inability to biosynthesize the accompanying enzyme clostripain. A process of obtaining the above mutant and its use in a process for the production of clostripain-free collagenase are also provided.

REFERENCES:
patent: 3201325 (1965-08-01), Barton
patent: 3677900 (1972-07-01), Merkel
patent: 3705083 (1972-12-01), Chiulli et al.
patent: 3821364 (1974-06-01), Chiulli et al.
Merck Index, 11th Edition, 1989, p. 1396 #8684 Soybean.
Emod, I. et al., Five sepharose bound ligands for the chromatog. pur. of Clostridium collagenase & clostripain (see abstract); 1977, 77/1 (51-56).

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