Mutant N -acetylglutamate synthase and method for...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

Reexamination Certificate

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C435S252330, C435S193000, C536S023200

Reexamination Certificate

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10891122

ABSTRACT:
L-arginine is produced using a bacterium belonging to the genusEscherichiaharboring a mutant N-acetylglutamate synthase in which the amino acid sequence corresponding to positions from 15 to 19 in a wild type N-acetylglutamate synthase is replaced with any one of amino acid sequences of SEQ ID NOS: 1 to 4, and feedback inhibition by L-arginine is desensitized.

REFERENCES:
patent: 1170361 (2002-09-01), None
C.F. Higgins, Annu. Rev. Cell Biol., vol. 8, pp. 67-113, “ABC Transporters: From Microorganisms to Man”, 1992.
T. Leisinger, et al., The Journal of Biological Chemistry, vol. 250, No. 5, pp. 1690-1693, “N-Acetylglutamate Synthase ofEscherichia coliRegulation of Synthesis and Activity by Arginine”, Mar. 10, 1975.
T. Takagi, et al., Biochem, vol. 99, No. 2, pp. 357-364, “Instability of an Arginine-Overproducing Mutant of Serratia Marcescents and its Stabilization”, 1986.
B.S. Rajagopal, et al., Applied and Enviromental Microbiology, vol. 64, No. 5, pp. 1805-1811, “Use of Inducible Feedback-Resistant N-Acetylglutamate Synthetase (argA) Genes for Enhanced Arginine Biosynthesis by Genetically EngineeredEscherichia coliK-12 Strains”, May 1998.
R. Cunn, et al., Microbiological Reviews, vol. 50, No. 3, pp. 314-358, XP-001068304, “Biosynthesis and Metabolism of Arginine in Bacteria”, Sep. 1986.
K. Brown, et al., Nucleic Acids Research, vol. 15, No. 24, p. 10586, XP-001063143, “Complete Nucleotide Sequence of theEscherichia coliArga Gene”, 1987.
V. Sakanyan, et al., Microbiology, vol. 142, No. 1, pp. 99-108, XP-001063142, “Genes and Enzymes of the Acetyl Cycle of Arginine Biosynthesis in Corynebacterium Glutamicum: Enzyme Evolution in the Early Steps of the Arginine Pathway”, 1996.
Hayashi et al. (Feb. 28, 2001) “Complete genome sequence of enterohemorrhagicEscherichia coliO157:H7 and genomic comparison with a laboratory strain K-12.” DNA Research, vol. 8, pp. 11-22.
Eckhardt et al. (1975) Molecular and General Genetics, vol. 138, No. 3, pp. 225-232.

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