Mutant microorganisms containing recombinant DNA and their use i

Details Mutant microorganisms containing recombinant DNA and their use i Mutant microorganisms containing recombinant DNA and their use i

1985-05-23

1989-02-21

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4351721, 4351723, 435202, 435839, 935 74, C12N 120, C12N 1500, C12N 928, C12R 1125

Patent

active

048064262

ABSTRACT:
Recombinant DNA containing amylase-coding genes is prepared by cleaving DNA from various donor microorganisms and combining portions of the DNA with the plasmid pUB110. Strains of E. coli or B. subtilis containing the recombinant DNA are grown in fermentation media to produce the amylase enzymes.

REFERENCES:
patent: 4469791 (1984-09-01), Colson et al.
patent: 4598048 (1986-07-01), Diderichsen et al.
patent: 4626510 (1986-12-01), Grandi
Takeichi et al., "Cloning of Bacillus subtilis .alpha.-Amylase Structural Gene in Plasmid pUB110", Agric. Biol. Chem. vol. 47, No. 1, (1983); pp. 159-161.
Ortlepp et al., "Molecular Cloning in Bacillus subtilis of a Bacillus licheniformis Gene Encoding a Thermostable Alpha Amylase", Gene, vol. 23, (1983); pp. 267-276.
Yamazaki et al., ".alpha.-Amylase Genes (amy R2 and dmyE.sup.+) from an .alpha.-Amylase-Hyperproducing Bacillus subtilis Strain:Molecular Cloning and Nucleotide Sequences"; J. Bacteriology, vol. 156, No. 1, (Oct. 1983); pp. 327-337.
Lehtovaara et al., "In vivo Transcription Initiation and Termination Sites of an .alpha.-Amylase Gene from Bacillus amyloliquefaciens Cloned in Bacillus subtilis", Gene, vol. 30, (Oct. 1984), pp. 11-16.
Ohmura et al.; "A Bacillus subtilis Secretion Vector System Derived from the B. subtilis .alpha.-Amylase Promotor and Signal Sequence Region, and Secretion of Escherichia coli .beta.-Lactamase by the Vector System"; J. Biochem, vol. 95 (1984); pp. 87-93.
Gryczan, et al., J. Bacteriology, 134, 318-329 (1978).
Palva, et al., Gene, 15, 43-51 (1981).
Palva, et al., Gene, 19, 81-87 (1982).
Stark, et al., FEMS Microbiol. Lett., 15, 295-298 (1982).

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