Mutant Lactobacillus bulgaricus strains free from...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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C435S253400, C424S439000, C424S780000, C426S042000, C426S043000

Reexamination Certificate

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06875601

ABSTRACT:
The invention concerns mutantL. bulgaricusstrains bearing a nonsense mutation, in at least one of the sequences coding for the lactose operon, and free from β-galactosidase activity, and lactic starters comprising said strains. Said strains and starters can be used to obtain fermented milk products from glucose-added milk.

REFERENCES:
patent: 5382438 (1995-01-01), Hottinger et al.
patent: 5639648 (1997-06-01), Mainzer et al.
patent: 5691185 (1997-11-01), Dickely et al.
patent: WO 90 05459 (1990-05-01), None
Yoast et al., “Generation and characterization of environmentally sensitive variants of the beta-galactosidase fromL. bulgaricus”, Applied and Environmental Microbiology, 1994, vol. 60, No. 4, p. 1221-1226—abstract.*
Mollet et al., “A beta-galactosidase deletion mutant ofL. bulgaricusreverts to generate an active enzyme by internal DNA seq duplication”, Mol Gen Genet, 1991, 227(1), 17-21—abstract.

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