Mutant human prourokinase

Details Mutant human prourokinase Mutant human prourokinase

1992-10-06

1995-02-14

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

424 9464, A61K 37547, C12N 972

Patent

active

053895380

ABSTRACT:
A mutant human prourokinase wherein a neutral amino acid in the epidermal growth factor (EGF) region of human prourokinase (human PUK) has been replaced with a basic amino acid, or an acidic amino acid has been replaced with a non-acidic amino acid, and a method for producing a mutant human PUK which comprises expression of mutant human PUK by cultivating a host transformed by a plasmid inserted with a DNA sequence coding for said mutant human PUK. By replacing a neutral amino acid in the EGF region of human PUK which is a fibrinolysin with a basic amino acid, or an acidic amino acid with a non-acidic amino acid, half-life in blood can be prolonged, and affinity for fibrin can be improved.

REFERENCES:
patent: 5098840 (1992-03-01), Kasai et al.
Proc. Natl. Acad. Sci. USA, "A GAL 10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site", Leonard Guarente et al., vol. 79, pp. 7410-7414, Dec. 1982.
Sobel, B. E. et al., Circulation 81:1362-1373 (1990).
Pierard, L. et al., J. Biotechnol. 15:283-304 (1990).
Lehninger, A. L., Principles of Biochemistry, Worth Publishers, Inc., New York (1982), pp. 100-108.
Hiramatsu, R. et al., Gene 99:235-241 (1991).
Li, X. K. et al., Biochim. Biophys. Acta 1159:37-43 (1992).
Lisnen, H. R. et al., Eur. J. Biochem. 205:701-709 (1992).

Affiliated with

Also associated with

Rating

Mutant human prourokinase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Mutant human prourokinase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mutant human prourokinase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-287300