Mutant GTP cyclohydrolase II enzymes

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...

Reexamination Certificate

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C435S004000, C435S006120, C435S069100, C435S071100, C435S252300, C435S195000, C435S440000, C435S320100, C536S023200

Reexamination Certificate

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07989189

ABSTRACT:
The present invention relates to modified GTP cyclohydrolase II enzymes that display increased specific activity, and to polynucleotides encoding them. The invention further pertains to vectors comprising these polynucleotides and host cells containing such vectors. The invention provides a method for producing the modified enzyme and a method for producing riboflavin, a riboflavin precursor, FMN, FAD, or a derivative thereof.

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Richter et al., “Biosynthesis of Riboflavin: Cloning, Sequencing, Mapping, and Expression of the Gene Coding for GTP Cyclohydrolase II inEscherichia coli,” J. Bact., vol. 175, No. 13, pp. 4045-4051, XP002095462 ISSN: 0021-9193 (1993).
Herz et al., “Biosynthesis of riboflavin in plants. The ribA gene ofArabidopsis thalianaspecifies a bifunctional GTP cyclohydrolase II/3,4-di-hydroxy-2-butanone 4-phosphate synthase,” Phytochem., vol. 53, No. 7, pp. 723-731, XP004291367 ISSN: 0031-9422 (2000).
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Bacher et al., “Biosynthesis of Riboflavin: GTP Cyclohydrolase II, Deaminase, And Reductase,” Methods in Enzymology, vol. 280, pp. 382-389, XP009030498 ISSN: 0076-6879 (1997).
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