Mutant gene causing a defect in mitochondrial recombination and

Details Mutant gene causing a defect in mitochondrial recombination and Mutant gene causing a defect in mitochondrial recombination and

1996-06-21

1999-06-01

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435449, 4353201, 536 235, 536 2431, C12Q 168, C07H 2104

Patent

active

059087471

ABSTRACT:
A multi-step method of detecting a gene in a nuclear chromosome which is involved in mitochondrial recombination is disclosed. The method disclosed includes the steps: a) fusing enucleated .omega..sup.- mitochondrial donor cells expressing a first marker gene with nucleus-containing .omega..sup.+ mitochondrial recipient cells expressing a second marker gene different from the first marker gene to form fused cells wherein the fused cells have one type of mitochondria and b) selecting fused cells that are .omega..sup.+ and express the first and second marker genes to identify those enucleated .omega..sup.- mitochondrial donor cells with a reduced recombination frequency. The method includes the further steps of judging that the gene in a nuclear chromosome of the recipient cells involved in mitochondrial recombination is normal when the mitochondrial recombination frequency is high or that the gene is mutated when the recombination frequency is low. The judging steps permit the detection of a mutant gene in a nuclear chromosome of the recipient cells used for cell fusion. An isolated mhr1 and MHR1 gene coding for a polypeptide is also disclosed.

REFERENCES:
Feng Ling et al., "A Nuclear Mutation Defective in Mitochondrial Recombination in Yeast," The EMBO Journal, vol. 14, No. 16, pp. 4090-4101, 1995.
Kei-ichi Nakagawa, et al., "An Endonuclease With Multiple Current Sites, Endo. Scel, Initiates Genetic Recombination at its Cutting Site in Yeast Mitochondria", The EMBO Journal, vol. 11, No. 7, pp. 2707-2715, 1992.
Helmut Bertrand, et al., "Hyperactive Recombination in the Mitochondrial DNA of the Natural Death Nuclear Mutant of Neurospora Crassa", Molecular and Cellular Biology, vol. 13, No. 11, pp. 6778-6788, 1993.

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