Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing a carotene nucleus
Reexamination Certificate
2008-09-09
2008-09-09
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing a carotene nucleus
C435S189000, C435S320100, C435S252300, C435S254100, C435S254200, C435S254300, C435S254110, C435S254600, C435S254210, C435S254220, C435S254230, C435S252310, C435S252320, C435S252330, C435S252340, C435S252350, C435S252200, C435S257200, C435S419000, C536S023200, C536S023400, C536S023700
Reexamination Certificate
active
11395644
ABSTRACT:
CrtW carotenoid ketolases are provided useful for the production of astaxanthin. The ketolases genes of the present invention exhibit improved ketolase activity when converting cyclic hydroxylated carotenoid intermediates into astaxanthin. Recombinant expression of the present carotenoid ketolases in host cell producing cyclic hydroxylated carotenoid intermediates enabled increased production of astaxanthin.
REFERENCES:
patent: 6150130 (2000-11-01), Misawa et al.
patent: 6291204 (2001-09-01), Pasamontes et al.
patent: 7074604 (2006-07-01), Tang et al.
patent: 2003/0087337 (2003-05-01), Giraud et al.
patent: 2005/0019852 (2005-01-01), Cheng et al.
patent: 2005/0214896 (2005-09-01), Cheng et al.
patent: WO 02/079395 (2002-10-01), None
patent: WO 2005/118812 (2005-12-01), None
patent: WO 2005/121352 (2005-12-01), None
CNCBI printout, Conserved domains of cd03513: CRTW—beta-carotene-ketolase, created Feb. 17, 2006.
Ye R. et al, Mutational and Functional Anlysis of the Beta-Crotene Ketolase Involved in the Production of Canthaxantin and Astaxanthin, Appl. Environ. Microbiol. 2006, 72, 5829-5837.
NCBI printout; GenBank Accession No. BAE47465, Nov. 1, 2005.
U.S. Appl. No. 11/015,433, filed Dec. 17, 2004, Qiong Cheng et al.
P. Bhosale et. al., Microbial Xanthophylls, PPL. Microbiol. Biotechnol., 2005, vol. 68:445-455.
Nelis et. al., Carotenoids From Micro-Organisms, Appl. Bacteriol., 1991, vol. 70:181-191.
N. Misawa et. al., Elucidation of theErwinia uredovoraCarotenoid Biosynthetic Pathway by Functional Analysis of Gene Products Expressed inEscherichia coli, J. Bacteriol., 1990, vol. 172:6704-6712.
N. Misawa et. al., Structure and Functional of a Marine Bacterial Carotenoid Biosynthesis Gene Cluster and Astaxanthin Biosynthetic Pathway Proposed at the Gene Level, J. Bacteriol., 1995, vol. 177:6575-6584.
Misawa et. al., Canthaxanthin Biosynthesis by the Conversion of Methylene to Keto Groups in a Hydrocarbon B-Carotene by a Single Gene, Biochem. Biophys. Res. Comm., 1995, vol. 209:867-876.
Hannibal; et. al., Isolation and Characterization of Canthaxanthin Biosynthesis Genes From the Photosynthetic BacteriumBradyrhizobiumso. Strain ORS278, J. Bacteriol., 2000, vol. 182:3850-3853.
Nishida et. al., Elucidation of a Carotenoid Biosynthesis Gene Cluster Encoding a Novel Enzyme, 2,2′-B-Hydroxylase, FromBrevundimonassp. Strain SD212 and Combinatorial Biosynthesis of New or Rare Xanthophylls, Appl. Env. Microbiol., 2005, vol. 71:4286-4296.
National Center for Biotechnology Information General Identifier No. 5912292, Sep. 15, 1999, M. Harker et. al., Carotenoid Biosynthesis Genes in the BacteriumParacoccus marcusii.
P. Fraser et. al., In Vitro Characterization of Astaxanthin Biosynthesis Enzymes, J. Biol. Chem., 1997, vol. 272:6128-6135.
P. Fraser et. al., Enzymic Confirmation of Reactions Involved in Routes to Astaxanthin Formation, Elucidated Using a Direct Substrate in Vitro Assay, Eur. J. Biochem., 1998, vol. 252:229-236.
Rick W. Ye et al., Mutational and Functional Analysis of the B-Carotene Ketolase Involved in the Production of Canthaxanthin and Astaxanthin, Applied and Environmental Microbiology, 2006, vol. 72:5829-5837.
International Search Report Dated Sep. 6, 2007, International Application No. PCT/US2007/006939, International Filing Date: Mar. 20, 2007.
Stead Kristen Janet
Yao Henry
Ye Rick W.
E.I. du Pont de Nemours and Company
Prouty Rebecca E.
Walicka Malgorzata A.
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