Mutant B-type DNA polymerases exhibiting improved...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120, C435S069100, C435S194000, C435S199000, C536S023100, C536S023200

Reexamination Certificate

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06881559

ABSTRACT:
The present invention relates to thermostable mutants of B-type DNA polymerases comprising a Y-GG/A amino acid motif between the N-terminal 3′-5′-exonuclease domain and the C-terminal polymerase domain whereas the tyrosine of the Y-GG/A amino acid motif is mutated and whereas these mutant DNA polymerases are suitable for PCR.

REFERENCES:
patent: 196 11 759 (1996-03-01), None
WF Anderson, Nature, “Human gene therapy”, Apr. 1998, vol. 392, pp. 25-28.*
G Antranikian et al., Accession DE19611759-A1, Apr. 1998.*
Veronica Truniger et al., Role of the “YxGG/A” Motif of 029 DNA Polymerase in Protein-primed Replication, J. Mol Biol. (1992). 286, pp. 57-69.*
Frank Niehaus et al., Cloning and characterisation of a thermostable x-DNA polymerase from the hyperthermophilic archaeonThermococcussp. TY, GENE 204 (1997), pp. 153-158.*
Veronica Truniger et al., A DNA binding motif coordinating synthesis and degradation in proofreading DNA polymerases. The Embo Journal, vol. 15, No. 13, pp. 3430-3441.*
Francesca M. Pisani et al., Amino Acid Residues Involved in Determining the Processivity of the 3′-5′ Exonuclease Activity in a Family B DNA Polymerase from the Thermoacidphilic ArchaeonSulfolobus solfataricus, Biochemistry 1998. 37, pp. 15005-15012.*
Domenico Bordo et al., Suggestions for “Safe” Residue Substiutions in Site-directed Mutagenisis, J. Mol. Biol. (1991). 217, pp. 721-729.*
Kristina Bohlke et al., PCR performance of the B-type DNA plymerase from the thermophilic euryachaeonThermococcus aggregansimproved by mutations in the Y-GG/A motif, Nucleic Acids Research, 2000. vol. 28. No. 20, pp. 3910-3917.

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